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• W2413307499 abstract "Summary A murine model of endothelial cell injury-based vascular thrombosis was used to test the role of platelet-endothelial cell adhesion molecule-1 (PECAM-1, CD31) in blood cell aggregate formation and vessel occlusion in vivo. Photochemically-induced thrombus formation was analyzed in detail using intravital fluorescence microscopy of individual microvessels in cremaster muscle preparations of CD31-deficient and wildtype mice. In venules, epi-illumination induced rapid thrombus formation with first platelet deposition after 0.56 ± 0.11min and complete vessel occlusion within 5.05 ± 0.45 min. In arterioles, thrombus formation was markedly delayed with first platelet deposition after 3.03 ± 0.47 min and complete vessel occlusion within 10.04 ± 1.26 min. Kinetics of thrombus formation in both venules (first platelet deposition: 0.52 ± 0.1 min; vessel occlusion: 5.03 ± 0.52 min) and arterioles (first platelet deposition: 3.06 ± 0.68 min; vessel occlusion: 10.02 ± 1.38 min) of CD31-deficient mice was found almost identical compared with that in wildtype animals. Tail bleeding time was 233 ± 24 s in wildtype and 243 ± 32 s in CD31-deficient mice. Moreover, CD31-deficient and wildtype mice revealed comparable interaction of leukocytes to endothelium. This study shows for the first time in vivo that CD31 is not critically involved in blood cell thrombus formation upon endothelial cell injury." @default.
• W2413307499 created "2016-06-24" @default.
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• W2413307499 date "2001-01-01" @default.
• W2413307499 modified "2023-10-16" @default.
• W2413307499 title "Lack of In Vivo Function of CD31 in Vascular Thrombosis" @default.
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• W2413307499 doi "https://doi.org/10.1055/s-0037-1612919" @default.
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