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• W2413307738 abstract "Vibrio parahaemolyticus is a leading cause of seafood-associated diarrhea and gastroenteritis. This bacteria expresses a major virulence determinant called T3SS1. Expression of T3SS1 is tightly regulated by the ExsA-ExsC-ExsD regulatory system. The transcription of exsA and probably exsC is repressed directly by the H-NS protein. In this study, the regulation of exsD by H-NS was investigated using quantitative RT-PCR, primer extension, LacZ fusion, electrophoretic mobility shift, and DNase I footprinting assays. The results showed that His-H-NS protected a single region from 61 bp to 174 bp upstream of exsD against DNase I digestion, and a transcription start site located at 105 bp upstream of exsD was detected and its activity was repressed by H-NS. Therefore, a single H-NS-dependent promoter was transcribed for exsD in V. parahaemolyticus. Thus, all three genes in the ExsA-ExsC-ExsD regulatory system of T3SS1 are directly repressed by H-NS in V. parahaemolyticus." @default.
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• W2413307738 date "2016-08-01" @default.
• W2413307738 modified "2023-10-03" @default.
• W2413307738 title "Transcription of exsD is repressed directly by H-NS in Vibrio parahaemolyticus" @default.
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• W2413307738 doi "https://doi.org/10.1016/j.micpath.2016.06.003" @default.
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