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- W2413373527 abstract "One of the problems that can occur in the development of durian (Durio zibethinus, L.) is the availability of seedlings and the method of propagation can cause over-exploitation of the mother plant. The aim of this research was to obtain the method of sterilization and the right media for in vitro propagation of durian. The research was carried out in the Tissue Culture Laboratory and Experimental Garden Faculty of Mathematics and Science, Yogyakarta State University. The method used in this research was induction using the cytokinin BAP (benzylamino purine) in MS media. The explants were obtained from durian seedlings that were grown in polybags with soil and compost media. Sterilization for the tissue culture technique was done using detergent, alcohol 70%, and clorox (20% and 10%), and also sterile aquadest for nodia, while leaf exsplant sterilization was done using clorox 10% and 5%. The media used were MS (Murashige and Skoog) with BAP (2 dan 4 ppm) for the nodia and also with 2,4-D (0.4, 1.0, dan 1.5 ppm) for the leaf explants. The result showed that the BAP in the MS media was able to induce the growth of shoots from the nodia at concentrations of 2 and 4 ppm. The addition of 2,4D was able to induce the growth of callus on the leaf explants. Using 1 ppm 2,4-D, the callus was white and transparent and the leaves did not curl so much as in the treatments with 0.4 and 1.5 ppm 2,4-D which caused the leaves to curl. More variation in the treatments or media both for the" @default.
- W2413373527 created "2016-06-24" @default.
- W2413373527 creator A5003155863 @default.
- W2413373527 date "2013-01-01" @default.
- W2413373527 modified "2023-09-23" @default.
- W2413373527 title "Eksplorasi Metode Sterilisasi dan Macam Media Untuk Perbanyakan Durian (Durio zibethinus, L.) Secara In Vitro (Exploration of Sterilization Method and Type of Media For In vitro Propagation of Durian (Durio zibethinus, L.))" @default.
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- W2413373527 hasPublicationYear "2013" @default.
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