FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2413435101> ?p ?o ?g. }

• W2413435101 endingPage "217" @default.
• W2413435101 startingPage "203" @default.
• W2413435101 abstract "Molecular machines and nanomotors are sophisticated biological assemblies that convert potential energy stored either in transmembrane ion gradients or in ATP into kinetic energy. Studying these highly dynamic biological devices by X-ray crystallography is challenging, as they are difficult to produce, purify, and crystallize. Phage display technology allows us to put a handle on these molecules in the form of highly specific antibody fragments that can also stabilize conformations and allow versatile labelling for electron microscopy, immunohistochemistry, and biophysics experiments.Here, we describe a widely applicable protocol for selecting high-affinity monoclonal antibody fragments against a complex molecular machine, the A-type ATPase from T. thermophilus that allows fast and simple purification of this transmembrane rotary motor from its wild-type source. The approach can be readily extended to other integral membrane proteins and protein complexes as well as to soluble molecular machines and nanomotors." @default.
• W2413435101 created "2016-06-24" @default.
• W2413435101 creator A5000689999 @default.
• W2413435101 creator A5043005286 @default.
• W2413435101 creator A5054944661 @default.
• W2413435101 date "2013-01-01" @default.
• W2413435101 modified "2023-09-27" @default.
• W2413435101 title "Purification of Molecular Machines and Nanomotors Using Phage-Derived Monoclonal Antibody Fragments" @default.
• W2413435101 cites W1969649052 @default.
• W2413435101 cites W1989639228 @default.
• W2413435101 cites W1990034705 @default.
• W2413435101 cites W1992860677 @default.
• W2413435101 cites W1995351026 @default.
• W2413435101 cites W2017051997 @default.
• W2413435101 cites W2045729352 @default.
• W2413435101 cites W2056485236 @default.
• W2413435101 cites W2061695953 @default.
• W2413435101 cites W2072945277 @default.
• W2413435101 cites W2083570386 @default.
• W2413435101 cites W2138461857 @default.
• W2413435101 cites W2140142521 @default.
• W2413435101 cites W2146941678 @default.
• W2413435101 cites W2147762950 @default.
• W2413435101 cites W2170937824 @default.
• W2413435101 cites W2171086036 @default.
• W2413435101 cites W2175687390 @default.
• W2413435101 doi "https://doi.org/10.1007/978-1-62703-354-1_12" @default.
• W2413435101 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23504426" @default.
• W2413435101 hasPublicationYear "2013" @default.
• W2413435101 type Work @default.
• W2413435101 sameAs 2413435101 @default.
• W2413435101 citedByCount "4" @default.
• W2413435101 countsByYear W24134351012014 @default.
• W2413435101 countsByYear W24134351012015 @default.
• W2413435101 countsByYear W24134351012017 @default.
• W2413435101 countsByYear W24134351012020 @default.
• W2413435101 crossrefType "book-chapter" @default.
• W2413435101 hasAuthorship W2413435101A5000689999 @default.
• W2413435101 hasAuthorship W2413435101A5043005286 @default.
• W2413435101 hasAuthorship W2413435101A5054944661 @default.
• W2413435101 hasConcept C12554922 @default.
• W2413435101 hasConcept C139425391 @default.
• W2413435101 hasConcept C144647389 @default.
• W2413435101 hasConcept C159654299 @default.
• W2413435101 hasConcept C170493617 @default.
• W2413435101 hasConcept C171250308 @default.
• W2413435101 hasConcept C185592680 @default.
• W2413435101 hasConcept C186268636 @default.
• W2413435101 hasConcept C192562407 @default.
• W2413435101 hasConcept C203014093 @default.
• W2413435101 hasConcept C24530287 @default.
• W2413435101 hasConcept C2779281246 @default.
• W2413435101 hasConcept C41625074 @default.
• W2413435101 hasConcept C542903549 @default.
• W2413435101 hasConcept C55493867 @default.
• W2413435101 hasConcept C70721500 @default.
• W2413435101 hasConcept C86803240 @default.
• W2413435101 hasConceptScore W2413435101C12554922 @default.
• W2413435101 hasConceptScore W2413435101C139425391 @default.
• W2413435101 hasConceptScore W2413435101C144647389 @default.
• W2413435101 hasConceptScore W2413435101C159654299 @default.
• W2413435101 hasConceptScore W2413435101C170493617 @default.
• W2413435101 hasConceptScore W2413435101C171250308 @default.
• W2413435101 hasConceptScore W2413435101C185592680 @default.
• W2413435101 hasConceptScore W2413435101C186268636 @default.
• W2413435101 hasConceptScore W2413435101C192562407 @default.
• W2413435101 hasConceptScore W2413435101C203014093 @default.
• W2413435101 hasConceptScore W2413435101C24530287 @default.
• W2413435101 hasConceptScore W2413435101C2779281246 @default.
• W2413435101 hasConceptScore W2413435101C41625074 @default.
• W2413435101 hasConceptScore W2413435101C542903549 @default.
• W2413435101 hasConceptScore W2413435101C55493867 @default.
• W2413435101 hasConceptScore W2413435101C70721500 @default.
• W2413435101 hasConceptScore W2413435101C86803240 @default.
• W2413435101 hasLocation W24134351011 @default.
• W2413435101 hasLocation W24134351012 @default.
• W2413435101 hasOpenAccess W2413435101 @default.
• W2413435101 hasPrimaryLocation W24134351011 @default.
• W2413435101 hasRelatedWork W1970625632 @default.
• W2413435101 hasRelatedWork W1973266002 @default.
• W2413435101 hasRelatedWork W2011201961 @default.
• W2413435101 hasRelatedWork W2046033225 @default.
• W2413435101 hasRelatedWork W2067571661 @default.
• W2413435101 hasRelatedWork W2092014932 @default.
• W2413435101 hasRelatedWork W2122559962 @default.
• W2413435101 hasRelatedWork W2123520905 @default.
• W2413435101 hasRelatedWork W2167786536 @default.
• W2413435101 hasRelatedWork W2276397704 @default.
• W2413435101 hasRelatedWork W2402662037 @default.
• W2413435101 hasRelatedWork W2409935913 @default.
• W2413435101 hasRelatedWork W2410486665 @default.
• W2413435101 hasRelatedWork W2418976081 @default.
• W2413435101 hasRelatedWork W2513614261 @default.
• W2413435101 hasRelatedWork W3152016270 @default.
• W2413435101 hasRelatedWork W3162954993 @default.
• W2413435101 hasRelatedWork W3175938140 @default.
• W2413435101 hasRelatedWork W3208568937 @default.
• W2413435101 hasRelatedWork W2168262853 @default.

• next