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• W2413594285 abstract "Glycosaminoglycans (GAGs), including heparan sulfate (HS), are expressed on the surface of nearly all cells, linked to transmembrane proteins. These GAGs are sulfated to varying extents, lending a negative charge, and are used by a large number of viruses to initiate infection of immortalized cell lines. Here we describe the rationale and methods for analyzing GAG usage by one such virus, respiratory syncytial virus (RSV). The protocols presented allow the determination of which GAG(s) is employed by the virus, which GAG modification(s) is important, and whether the important GAG is on the cell or on the virus. We also discuss the finding that many viruses are selected for GAG usage during passage in culture and present a method for rapidly determining whether GAG usage is characteristic of a wild virus or is limited to laboratory-adapted virus." @default.
• W2413594285 created "2016-06-24" @default.
• W2413594285 creator A5018978935 @default.
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• W2413594285 date "2007-01-01" @default.
• W2413594285 modified "2023-10-06" @default.
• W2413594285 title "Interaction Between Respiratory Syncytial Virus and Glycosaminoglycans, Including Heparan Sulfate" @default.
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• W2413594285 doi "https://doi.org/10.1007/978-1-59745-393-6_2" @default.
• W2413594285 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17502668" @default.
• W2413594285 hasPublicationYear "2007" @default.
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