FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2413642973> ?p ?o ?g. }

• W2413642973 endingPage "103" @default.
• W2413642973 startingPage "91" @default.
• W2413642973 abstract "The murine embryonal teratocarcinoma cell line, P19, was genetically manipulated in order to provide preliminary information on compounds that induce differentiation. Without chemical induction, P19 cells remain in an undifferentiated state, but can be induced to differentiate into specific cell types. For example, dimethyl sulphoxide (DMSO) induces cardiac and skeletal muscle differentiation, whereas retinoic acid stimulates neuronal differentiation. P19 cells were transfected with a construct containing a segment of the murineTert (mTert) promoter sequence combined with the green fluorescent protein (GFP) gene, which acts as a reporter gene. mTert expression, the reverse transcriptase component of murine telomerase, is closely linked to telomerase activity and is down-regulated during differentiation. Three retinoids and DMSO induced the differentiation of P19 cells, which was determined by a reduction in mTert_GFP expression, detected by flow cytometry and confocal microscopy as independent methods of detection. A test substance, ethanol, and a control substance, saccharin, did not cause a decrease in mTert_GFP expression. In addition, it could be demonstrated that the mTert_GFP test detects developmentally relevant effects at non-cytotoxic concentrations. The ID50 values derived for the reduction of mTert_GFP expression were lower than the IC50 values detected with the MTT test, by a factor of 21.4 for all-trans retinoic acid, 12.7 for 9-cis retinoic acid, 29.6 for 13-cis retinoic acid, and 8.7 for DMSO. In comparison to the IC50 value for the P19 cell line, a similar IC50 value was obtained with 3T3 cells for ethanol, but there was a 2-fold increase for DMSO. The retinoids were not cytotoxic to 3T3 cells at the concentrations tested. This newly developed test is capable of detecting differentiation-inducing compounds at non-cytotoxic concentrations within 4 days. It offers a method for detecting chemicals with specific toxicological mechanisms, such as the retinoids, which could provide additional information in embryotoxicity testing as different promoters could be employed. Here, we report the use of this novel test system for the successful analysis of DMSO and three retinoids with different in vivo teratogenic potentials." @default.
• W2413642973 created "2016-06-24" @default.
• W2413642973 creator A5019894906 @default.
• W2413642973 creator A5053822823 @default.
• W2413642973 creator A5060528153 @default.
• W2413642973 creator A5074717230 @default.
• W2413642973 creator A5091124551 @default.
• W2413642973 date "2005-04-01" @default.
• W2413642973 modified "2023-10-16" @default.
• W2413642973 title "The Detection of Differentiation-inducing Chemicals by using Green Fluorescent Protein Expression in Genetically Engineered Teratocarcinoma Cells" @default.
• W2413642973 cites W104300299 @default.
• W2413642973 cites W1981457253 @default.
• W2413642973 cites W1983991725 @default.
• W2413642973 cites W1985800918 @default.
• W2413642973 cites W1989106487 @default.
• W2413642973 cites W1998245693 @default.
• W2413642973 cites W1999468613 @default.
• W2413642973 cites W2002810221 @default.
• W2413642973 cites W2013693833 @default.
• W2413642973 cites W2013851601 @default.
• W2413642973 cites W2016980442 @default.
• W2413642973 cites W2017101000 @default.
• W2413642973 cites W2017578370 @default.
• W2413642973 cites W2022507419 @default.
• W2413642973 cites W2026345971 @default.
• W2413642973 cites W2027815400 @default.
• W2413642973 cites W2039651932 @default.
• W2413642973 cites W2043195815 @default.
• W2413642973 cites W2050467330 @default.
• W2413642973 cites W2066758609 @default.
• W2413642973 cites W2078691986 @default.
• W2413642973 cites W2079042280 @default.
• W2413642973 cites W2081178187 @default.
• W2413642973 cites W2083061280 @default.
• W2413642973 cites W2089327546 @default.
• W2413642973 cites W2089389307 @default.
• W2413642973 cites W2089443863 @default.
• W2413642973 cites W2093308617 @default.
• W2413642973 cites W2095368297 @default.
• W2413642973 cites W2098470464 @default.
• W2413642973 cites W2114811563 @default.
• W2413642973 cites W2126908105 @default.
• W2413642973 cites W2144359607 @default.
• W2413642973 cites W2146986989 @default.
• W2413642973 cites W2154158394 @default.
• W2413642973 cites W2158343596 @default.
• W2413642973 cites W2170777028 @default.
• W2413642973 cites W2217888991 @default.
• W2413642973 cites W4251506604 @default.
• W2413642973 doi "https://doi.org/10.1177/026119290503300204" @default.
• W2413642973 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16180984" @default.
• W2413642973 hasPublicationYear "2005" @default.
• W2413642973 type Work @default.
• W2413642973 sameAs 2413642973 @default.
• W2413642973 citedByCount "7" @default.
• W2413642973 countsByYear W24136429732017 @default.
• W2413642973 countsByYear W24136429732018 @default.
• W2413642973 countsByYear W24136429732023 @default.
• W2413642973 crossrefType "journal-article" @default.
• W2413642973 hasAuthorship W2413642973A5019894906 @default.
• W2413642973 hasAuthorship W2413642973A5053822823 @default.
• W2413642973 hasAuthorship W2413642973A5060528153 @default.
• W2413642973 hasAuthorship W2413642973A5074717230 @default.
• W2413642973 hasAuthorship W2413642973A5091124551 @default.
• W2413642973 hasBestOaLocation W24136429731 @default.
• W2413642973 hasConcept C104317684 @default.
• W2413642973 hasConcept C125593758 @default.
• W2413642973 hasConcept C142613039 @default.
• W2413642973 hasConcept C148738053 @default.
• W2413642973 hasConcept C153911025 @default.
• W2413642973 hasConcept C185592680 @default.
• W2413642973 hasConcept C20875687 @default.
• W2413642973 hasConcept C26805974 @default.
• W2413642973 hasConcept C2781121885 @default.
• W2413642973 hasConcept C2908788973 @default.
• W2413642973 hasConcept C54009773 @default.
• W2413642973 hasConcept C54355233 @default.
• W2413642973 hasConcept C55493867 @default.
• W2413642973 hasConcept C81885089 @default.
• W2413642973 hasConcept C86803240 @default.
• W2413642973 hasConcept C94581717 @default.
• W2413642973 hasConcept C95444343 @default.
• W2413642973 hasConceptScore W2413642973C104317684 @default.
• W2413642973 hasConceptScore W2413642973C125593758 @default.
• W2413642973 hasConceptScore W2413642973C142613039 @default.
• W2413642973 hasConceptScore W2413642973C148738053 @default.
• W2413642973 hasConceptScore W2413642973C153911025 @default.
• W2413642973 hasConceptScore W2413642973C185592680 @default.
• W2413642973 hasConceptScore W2413642973C20875687 @default.
• W2413642973 hasConceptScore W2413642973C26805974 @default.
• W2413642973 hasConceptScore W2413642973C2781121885 @default.
• W2413642973 hasConceptScore W2413642973C2908788973 @default.
• W2413642973 hasConceptScore W2413642973C54009773 @default.
• W2413642973 hasConceptScore W2413642973C54355233 @default.
• W2413642973 hasConceptScore W2413642973C55493867 @default.
• W2413642973 hasConceptScore W2413642973C81885089 @default.
• W2413642973 hasConceptScore W2413642973C86803240 @default.
• W2413642973 hasConceptScore W2413642973C94581717 @default.

• next