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- W2413801860 abstract "Fourteen membranes out of cellulose (Cuprophan R ), polyamide and polypropylene were compared in a cytocompatibility test using the cytokinetics and cytomorphology of primary hepatatocytes as parameters. Additionally, the impact of coating the membranes with collagen or fibronectin was investigated. Hepatocytes were not able to attach in acceptable amounts on investigated cellulose membranes. On polyamide and polypropylene membranes a sufficient cell seeding was possible. Coating with collagen or fibronectin improves the attachment and spreading on all membranes. Differences between collagen and fibronectin were detected, observing the morphology of the cells: on collagen, most of the cells spread, whilst on fibronectin, most of the cells spread and flattened polygonally. If the adhesion of hepatocytes prolongs their metabolic function, a large adhesion surface in bioreactors is necessary. To reach a high surface area for cell adhesion in bioreactors one possibility is the use of polyamide and polypropylene membranes." @default.
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- W2413801860 date "1990-07-01" @default.
- W2413801860 modified "2023-09-26" @default.
- W2413801860 title "Membranes as Substrates for Hepatocyte Adhesion in Liver Support Bioreactors" @default.
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- W2413801860 doi "https://doi.org/10.1177/039139889001300710" @default.
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