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- W2413821479 abstract "In this work, a SYBR Green I real-time PCR method has been developed for the detection and quantification of Aspergillus carbonarius in grapes by targeting the veA gene with a primer pair (veAF4/veAR4) that specifically amplifies a 91-bp PCR product. The quantification of the fungal DNA was performed by generation of standard curves for two A. carbonarius strains, using spectrophotometrically measured DNA quantities (Log) with a linearity range from 50 to 5 × 10(-4) ng of DNA. A high positive correlation (R(2) > 0.99) between exponential increases of DNA and real-time PCR threshold cycles showed a high amplification efficiency for the assay (E values 100.06 and 101.51%, respectively). Quantification of the fungal genomic DNA in grape samples artificially inoculated with A. carbonarius conidia was successfully performed with a minimum threshold of 10(4) conidia per g of grape berry. The assay developed would allow reliable, specific, and efficient detection and quantification of A. carbonarius in grapes." @default.
- W2413821479 created "2016-06-24" @default.
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- W2413821479 date "2015-12-01" @default.
- W2413821479 modified "2023-09-23" @default.
- W2413821479 title "Real-Time PCR Assay Targeting the Gene for Quantification of in Grapes" @default.
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- W2413821479 doi "https://doi.org/10.4315/0362-028x.jfp-15-065" @default.
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