Matches in SemOpenAlex for { <https://semopenalex.org/work/W2413951306> ?p ?o ?g. }
Showing items 1 to 90 of
90
with 100 items per page.
- W2413951306 endingPage "2857" @default.
- W2413951306 startingPage "2853" @default.
- W2413951306 abstract "Objective To explore the feasibility of inhibition of hTERT and androgen receptor (AR) gene expression simultaneously in LNCaP cells by single shRNA vector.Methods Templates DNA of beth hTERT and AR siRNA were inserted into Pgenesil vector to construct a new vector Pgenesil-hTERT-AR-shRNA by RNAi-DNA vector technology.Pgenesil-HK-shRNA,Pgenesil-hTERT-shRNA,Pgenesil-AR-shRNA and Pgenesil-hTERT-AR-shRNA vectors were transfected into prostate cancer LNCaP cells respectively.The levels of AR mRNA,apoptosis and proliferation of each cell group were determined by FQPCR,Annexin V method and MTT.Results The level of hTERT mRNA of control group cells and cells transfected by Pgenesil-HK-shRNA,Pgenesil-hTERT-shRNA,Pgenesil-AR-shRNA and Pgenesil-hTERT-AR-shRNA was (1.51±0.08)×108,(7.32±0.43)×107,(2.94±0.15)×106,(4.45±0.25)×107 and(3.17±0.18)×106 (copies/ml) respectively.The level of AR mRNA of control cell groups and cells transfected by Pgenesil-HK-shRNA,Pgenesii-hTERT-shRNA,Pgenesil-AR-shRNA and Pgenesil-hTERT-AR-shRNA was (1.92±0.11)×105,(6.47±0.32)×105,(3.70±0.24)×104,(1.22±0.06)×104 and (7.21±0.41)×103 (copies/ml) respectively.These data indicate that the expression of hTERT or AR gene could be significantly inhibited by Pgenesil-hTERT-shRNA or Pgenesil-AR-shRNA while Pgenosil-hTERT-AR-shRNA could simultaneously inhibit both hTERT and AR gene expression.The apoptosis rate and the inhibition rate of cell growth of Pgenesil-hTERT-AR-shRNA group were significantly higher than those of Pgenesil-hTERT-shRNA group or Pgenesil-AR-shRNA group (P＜0.05).Conclusion It is feasible to inhibit both hTERT and AR gene expression simultaneously by single shRNA vector.It will be a new research strategy of gene therapy for prostate cancer.Key words: Prostate cancer; Human telomerase reverse n-anscriptase (hTERT); Androgen receptor; RNA interference" @default.
- W2413951306 created "2016-06-24" @default.
- W2413951306 creator A5002960926 @default.
- W2413951306 creator A5004004656 @default.
- W2413951306 creator A5055814924 @default.
- W2413951306 creator A5081086027 @default.
- W2413951306 date "2009-11-03" @default.
- W2413951306 modified "2023-09-28" @default.
- W2413951306 title "Simultaneous inhibition of both hTERT and androgen receptor gene expression in LNCaP cells using single shRNA vector" @default.
- W2413951306 doi "https://doi.org/10.3760/cma.j.issn.0376-2491.2009.40.013" @default.
- W2413951306 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20137668" @default.
- W2413951306 hasPublicationYear "2009" @default.
- W2413951306 type Work @default.
- W2413951306 sameAs 2413951306 @default.
- W2413951306 citedByCount "0" @default.
- W2413951306 crossrefType "journal-article" @default.
- W2413951306 hasAuthorship W2413951306A5002960926 @default.
- W2413951306 hasAuthorship W2413951306A5004004656 @default.
- W2413951306 hasAuthorship W2413951306A5055814924 @default.
- W2413951306 hasAuthorship W2413951306A5081086027 @default.
- W2413951306 hasConcept C104317684 @default.
- W2413951306 hasConcept C121608353 @default.
- W2413951306 hasConcept C125593758 @default.
- W2413951306 hasConcept C153911025 @default.
- W2413951306 hasConcept C166703698 @default.
- W2413951306 hasConcept C173396325 @default.
- W2413951306 hasConcept C190232843 @default.
- W2413951306 hasConcept C190283241 @default.
- W2413951306 hasConcept C2779723316 @default.
- W2413951306 hasConcept C2780192828 @default.
- W2413951306 hasConcept C54009773 @default.
- W2413951306 hasConcept C54355233 @default.
- W2413951306 hasConcept C55493867 @default.
- W2413951306 hasConcept C61367390 @default.
- W2413951306 hasConcept C67705224 @default.
- W2413951306 hasConcept C81885089 @default.
- W2413951306 hasConcept C86803240 @default.
- W2413951306 hasConcept C94581717 @default.
- W2413951306 hasConcept C96232424 @default.
- W2413951306 hasConceptScore W2413951306C104317684 @default.
- W2413951306 hasConceptScore W2413951306C121608353 @default.
- W2413951306 hasConceptScore W2413951306C125593758 @default.
- W2413951306 hasConceptScore W2413951306C153911025 @default.
- W2413951306 hasConceptScore W2413951306C166703698 @default.
- W2413951306 hasConceptScore W2413951306C173396325 @default.
- W2413951306 hasConceptScore W2413951306C190232843 @default.
- W2413951306 hasConceptScore W2413951306C190283241 @default.
- W2413951306 hasConceptScore W2413951306C2779723316 @default.
- W2413951306 hasConceptScore W2413951306C2780192828 @default.
- W2413951306 hasConceptScore W2413951306C54009773 @default.
- W2413951306 hasConceptScore W2413951306C54355233 @default.
- W2413951306 hasConceptScore W2413951306C55493867 @default.
- W2413951306 hasConceptScore W2413951306C61367390 @default.
- W2413951306 hasConceptScore W2413951306C67705224 @default.
- W2413951306 hasConceptScore W2413951306C81885089 @default.
- W2413951306 hasConceptScore W2413951306C86803240 @default.
- W2413951306 hasConceptScore W2413951306C94581717 @default.
- W2413951306 hasConceptScore W2413951306C96232424 @default.
- W2413951306 hasIssue "40" @default.
- W2413951306 hasLocation W24139513061 @default.
- W2413951306 hasLocation W24139513062 @default.
- W2413951306 hasOpenAccess W2413951306 @default.
- W2413951306 hasPrimaryLocation W24139513061 @default.
- W2413951306 hasRelatedWork W2072341838 @default.
- W2413951306 hasRelatedWork W2073821332 @default.
- W2413951306 hasRelatedWork W2347918426 @default.
- W2413951306 hasRelatedWork W2348722706 @default.
- W2413951306 hasRelatedWork W2351575159 @default.
- W2413951306 hasRelatedWork W2355401262 @default.
- W2413951306 hasRelatedWork W2360566849 @default.
- W2413951306 hasRelatedWork W2366726948 @default.
- W2413951306 hasRelatedWork W2371985867 @default.
- W2413951306 hasRelatedWork W2373265842 @default.
- W2413951306 hasRelatedWork W2378129852 @default.
- W2413951306 hasRelatedWork W2380158255 @default.
- W2413951306 hasRelatedWork W2385849154 @default.
- W2413951306 hasRelatedWork W2389240919 @default.
- W2413951306 hasRelatedWork W2390609086 @default.
- W2413951306 hasRelatedWork W2841394358 @default.
- W2413951306 hasRelatedWork W3030580256 @default.
- W2413951306 hasRelatedWork W3030595621 @default.
- W2413951306 hasRelatedWork W3031650065 @default.
- W2413951306 hasRelatedWork W3143928530 @default.
- W2413951306 hasVolume "89" @default.
- W2413951306 isParatext "false" @default.
- W2413951306 isRetracted "false" @default.
- W2413951306 magId "2413951306" @default.
- W2413951306 workType "article" @default.