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- W2414012011 abstract "Glycyrrhizin is an important bioactive compound that is used clinically to treat chronic hepatitis and is also used as a sweetener world-wide. However, the key UDP-dependent glucuronosyltransferases (UGATs) involved in the biosynthesis of glycyrrhizin remain unknown. To discover unknown UGATs, we fully annotated potential UGATs from Glycyrrhiza uralensis using deep transcriptome sequencing. The catalytic functions of candidate UGATs were determined by an in vitro enzyme assay. Systematically screening 434 potential UGATs, we unexpectedly found one unique GuUGAT that was able to catalyse the glucuronosylation of glycyrrhetinic acid to directly yield glycyrrhizin via continuous two-step glucuronosylation. Expression analysis further confirmed the key role of GuUGAT in the biosynthesis of glycyrrhizin. Site-directed mutagenesis revealed that Gln-352 may be important for the initial step of glucuronosylation, and His-22, Trp-370, Glu-375 and Gln-392 may be important residues for the second step of glucuronosylation. Notably, the ability of GuUGAT to catalyse a continuous two-step glucuronosylation reaction was determined to be unprecedented among known glycosyltransferases of bioactive plant natural products. Our findings increase the understanding of traditional glycosyltransferases and pave the way for the complete biosynthesis of glycyrrhizin." @default.
- W2414012011 created "2016-06-24" @default.
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- W2414012011 date "2016-06-02" @default.
- W2414012011 modified "2023-10-16" @default.
- W2414012011 title "A novel glucuronosyltransferase has an unprecedented ability to catalyse continuous two‐step glucuronosylation of glycyrrhetinic acid to yield glycyrrhizin" @default.
- W2414012011 cites W1172908466 @default.
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- W2414012011 cites W1635829456 @default.
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- W2414012011 cites W1874287518 @default.
- W2414012011 cites W1916210473 @default.
- W2414012011 cites W1965912922 @default.
- W2414012011 cites W1969657382 @default.
- W2414012011 cites W1977287866 @default.
- W2414012011 cites W1977828927 @default.
- W2414012011 cites W1982987035 @default.
- W2414012011 cites W1987433712 @default.
- W2414012011 cites W1989253463 @default.
- W2414012011 cites W1995631830 @default.
- W2414012011 cites W1999574084 @default.
- W2414012011 cites W2005476163 @default.
- W2414012011 cites W2006680168 @default.
- W2414012011 cites W2010122020 @default.
- W2414012011 cites W2015925567 @default.
- W2414012011 cites W2018289835 @default.
- W2414012011 cites W2021337699 @default.
- W2414012011 cites W2025191472 @default.
- W2414012011 cites W2025410007 @default.
- W2414012011 cites W2029157859 @default.
- W2414012011 cites W2036595566 @default.
- W2414012011 cites W2037722188 @default.
- W2414012011 cites W2039043591 @default.
- W2414012011 cites W2044280032 @default.
- W2414012011 cites W2047841570 @default.
- W2414012011 cites W2052567898 @default.
- W2414012011 cites W2055297061 @default.
- W2414012011 cites W2065439398 @default.
- W2414012011 cites W2070429529 @default.
- W2414012011 cites W2070550618 @default.
- W2414012011 cites W2077182467 @default.
- W2414012011 cites W2079094059 @default.
- W2414012011 cites W2081417228 @default.
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- W2414012011 cites W2100450322 @default.
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- W2414012011 cites W2101921145 @default.
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- W2414012011 cites W2114384345 @default.
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- W2414012011 cites W2129586833 @default.
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- W2414012011 cites W2286908285 @default.
- W2414012011 cites W2334838144 @default.
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- W2414012011 doi "https://doi.org/10.1111/nph.14039" @default.
- W2414012011 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7167757" @default.
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