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- W2414146602 abstract "Deadenylation is the removal of poly(A) tails from mRNA. This chapter presents two methods to assay deadenylation in vitro. The first is a quick and quantitative assay for the degradation of radiolabeled poly(A) that can easily be adapted to be used for many different enzymes. The second method uses an extract from Drosophila embryos to catalyze the deadenylation of an RNA dependent on a specific sequence that also directs deadenylation in vivo." @default.
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- W2414146602 date "2014-01-01" @default.
- W2414146602 modified "2023-10-17" @default.
- W2414146602 title "Assaying mRNA Deadenylation In Vitro" @default.
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- W2414146602 doi "https://doi.org/10.1007/978-1-62703-971-0_24" @default.
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