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- W2414149710 abstract "Linderstr#{248}m-Lang and Holter quantitative histochemical method (5, 4), as extended by Lowry (6). The advantages and limitations of the modified method have been discussed elsewhere (13, 16). Other authors in this symposium will review staining histochemical methods (Sidman), the use of other quantitative methods for localization (Giacobini), and the application of the classical quantitative histochemical methods to the cerebral cortex (Pope). Although the steps in the modified method have been described (6), it is convenient to summarize them here: (1) A piece of tissue approximately 0.25 cm3 is frozen in liquid nitrogen or in Freon 12 chified in liquid nitrogen. (2) The tissue is allowed to warm to -20#{176}C, mounted for sectioning in a microtome, and sections (6-50 ) are cut at 14#{176}C to -35#{176}Cin a cryostat. The individual sections are collected in special holders and placed for dehydration in a glass tube fitted with a stopcock. (3) The sections are dehydrated overnight at -40#{176}C in a vacuum of 0.05 mm Hg or less. (4) The tissue is then warmed to room temperature while still under vacuum. Selected areas weighing 0.002 to 20 ig are cut or teased from the unstained sections under a dissecting microscope at 40to 90-fold magnification. The dissection is done manually, using a sharp scalpel, broken-off razor blades, or sharpened needles. (5) The dissected specimens are weighed on a “fishpole” balance and placed in test tubes made from Pyrex tubing. (6) Chemical analyses are then performed by colorimetry or fluorimetry. * Aided by grants from The National Foundation and the National Multiple Sclerosis Society. The special modifications necessary to analyze individual cell bodies have been described (8)." @default.
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- W2414149710 date "1960-11-01" @default.
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- W2414149710 title "THE CHEMICAL COMPOSITION OF CENTRAL TRACTS AND OF NERVE CELL BODIES" @default.
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- W2414149710 doi "https://doi.org/10.1177/8.6.431" @default.
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