Matches in SemOpenAlex for { <https://semopenalex.org/work/W2414196175> ?p ?o ?g. }
Showing items 1 to 99 of
99
with 100 items per page.
- W2414196175 endingPage "191" @default.
- W2414196175 startingPage "175" @default.
- W2414196175 abstract "Knowledge of an individual protein's modifications, binding partners, and localization is essential for understanding complex biological networks. We recently described a fluorescent protein-based (mVenus) multifunctional affinity purification (MAP) tag that can be used both to purify a given protein and determine its localization (Ma et al., Mol Cell Proteomics 11:501-511, 2012). MAP purified protein complexes can be further analyzed to identify binding partners and posttranslational modifications by LC-MS/MS. The MAP approach offers rapid FACS-selection of stable clonal cell lines based on the expression level/fluorescence of the MAP-protein fusion. The MAP tag is highly efficient and shows little variability between proteins. Here we describe the general MAP purification method in detail, and show how it can be applied to a specific protein using the human Cdc14B phosphatase as an example." @default.
- W2414196175 created "2016-06-24" @default.
- W2414196175 creator A5038071740 @default.
- W2414196175 creator A5040815798 @default.
- W2414196175 creator A5066662711 @default.
- W2414196175 creator A5079966045 @default.
- W2414196175 date "2014-01-01" @default.
- W2414196175 modified "2023-10-04" @default.
- W2414196175 title "An Efficient Fluorescent Protein-Based Multifunctional Affinity Purification Approach in Mammalian Cells" @default.
- W2414196175 cites W1575542444 @default.
- W2414196175 cites W1990273811 @default.
- W2414196175 cites W1991518289 @default.
- W2414196175 cites W1996321460 @default.
- W2414196175 cites W2013412105 @default.
- W2414196175 cites W2029553332 @default.
- W2414196175 cites W2052168634 @default.
- W2414196175 cites W2053573662 @default.
- W2414196175 cites W2064355142 @default.
- W2414196175 cites W2068749491 @default.
- W2414196175 cites W2072619431 @default.
- W2414196175 cites W2098715685 @default.
- W2414196175 cites W2108025706 @default.
- W2414196175 cites W2109729417 @default.
- W2414196175 cites W2114958077 @default.
- W2414196175 cites W2133561717 @default.
- W2414196175 cites W2143094150 @default.
- W2414196175 cites W2155142785 @default.
- W2414196175 cites W2164400454 @default.
- W2414196175 cites W2167060626 @default.
- W2414196175 doi "https://doi.org/10.1007/978-1-4939-1034-2_14" @default.
- W2414196175 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24943323" @default.
- W2414196175 hasPublicationYear "2014" @default.
- W2414196175 type Work @default.
- W2414196175 sameAs 2414196175 @default.
- W2414196175 citedByCount "2" @default.
- W2414196175 countsByYear W24141961752020 @default.
- W2414196175 crossrefType "book-chapter" @default.
- W2414196175 hasAuthorship W2414196175A5038071740 @default.
- W2414196175 hasAuthorship W2414196175A5040815798 @default.
- W2414196175 hasAuthorship W2414196175A5066662711 @default.
- W2414196175 hasAuthorship W2414196175A5079966045 @default.
- W2414196175 hasConcept C104317684 @default.
- W2414196175 hasConcept C121332964 @default.
- W2414196175 hasConcept C123894998 @default.
- W2414196175 hasConcept C142613039 @default.
- W2414196175 hasConcept C153911025 @default.
- W2414196175 hasConcept C179247698 @default.
- W2414196175 hasConcept C181199279 @default.
- W2414196175 hasConcept C185592680 @default.
- W2414196175 hasConcept C30417494 @default.
- W2414196175 hasConcept C40767141 @default.
- W2414196175 hasConcept C46111723 @default.
- W2414196175 hasConcept C55493867 @default.
- W2414196175 hasConcept C62520636 @default.
- W2414196175 hasConcept C70721500 @default.
- W2414196175 hasConcept C71095615 @default.
- W2414196175 hasConcept C86803240 @default.
- W2414196175 hasConcept C8985478 @default.
- W2414196175 hasConcept C91881484 @default.
- W2414196175 hasConcept C95444343 @default.
- W2414196175 hasConceptScore W2414196175C104317684 @default.
- W2414196175 hasConceptScore W2414196175C121332964 @default.
- W2414196175 hasConceptScore W2414196175C123894998 @default.
- W2414196175 hasConceptScore W2414196175C142613039 @default.
- W2414196175 hasConceptScore W2414196175C153911025 @default.
- W2414196175 hasConceptScore W2414196175C179247698 @default.
- W2414196175 hasConceptScore W2414196175C181199279 @default.
- W2414196175 hasConceptScore W2414196175C185592680 @default.
- W2414196175 hasConceptScore W2414196175C30417494 @default.
- W2414196175 hasConceptScore W2414196175C40767141 @default.
- W2414196175 hasConceptScore W2414196175C46111723 @default.
- W2414196175 hasConceptScore W2414196175C55493867 @default.
- W2414196175 hasConceptScore W2414196175C62520636 @default.
- W2414196175 hasConceptScore W2414196175C70721500 @default.
- W2414196175 hasConceptScore W2414196175C71095615 @default.
- W2414196175 hasConceptScore W2414196175C86803240 @default.
- W2414196175 hasConceptScore W2414196175C8985478 @default.
- W2414196175 hasConceptScore W2414196175C91881484 @default.
- W2414196175 hasConceptScore W2414196175C95444343 @default.
- W2414196175 hasLocation W24141961751 @default.
- W2414196175 hasLocation W24141961752 @default.
- W2414196175 hasOpenAccess W2414196175 @default.
- W2414196175 hasPrimaryLocation W24141961751 @default.
- W2414196175 hasRelatedWork W2095373397 @default.
- W2414196175 hasRelatedWork W2130171154 @default.
- W2414196175 hasRelatedWork W2140671887 @default.
- W2414196175 hasRelatedWork W2253274787 @default.
- W2414196175 hasRelatedWork W2293347756 @default.
- W2414196175 hasRelatedWork W2572123960 @default.
- W2414196175 hasRelatedWork W2941574532 @default.
- W2414196175 hasRelatedWork W3185223799 @default.
- W2414196175 hasRelatedWork W4210666139 @default.
- W2414196175 hasRelatedWork W2018503930 @default.
- W2414196175 isParatext "false" @default.
- W2414196175 isRetracted "false" @default.
- W2414196175 magId "2414196175" @default.
- W2414196175 workType "book-chapter" @default.