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- W2414271346 abstract "Analytical ultracentrifugation (AUC) is a powerful tool that can provide thermodynamic information on associating systems. Here, we discuss how to use the two fundamental AUC applications, sedimentation velocity (SV), and sedimentation equilibrium (SE), to study nonspecific protein–nucleic acid interactions, with a special emphasis on how to analyze the experimental data to extract thermodynamic information. We discuss three specific applications of this approach: (i) determination of nonspecific binding stoichiometry of E. coli integration host factor protein to dsDNA, (ii) characterization of nonspecific binding properties of Adenoviral IVa2 protein to dsDNA using SE-AUC, and (iii) analysis of the competition between specific and nonspecific DNA-binding interactions observed for E. coli integration host factor protein assembly on dsDNA. These approaches provide powerful tools that allow thermodynamic interrogation and thus a mechanistic understanding of how proteins bind nucleic acids by both specific and nonspecific interactions." @default.
- W2414271346 created "2016-06-24" @default.
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- W2414271346 date "2015-01-01" @default.
- W2414271346 modified "2023-09-23" @default.
- W2414271346 title "Analytical Ultracentrifugation as a Tool to Study Nonspecific Protein–DNA Interactions" @default.
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- W2414271346 doi "https://doi.org/10.1016/bs.mie.2015.04.009" @default.
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