Matches in SemOpenAlex for { <https://semopenalex.org/work/W2414278897> ?p ?o ?g. }
Showing items 1 to 78 of
78
with 100 items per page.
- W2414278897 endingPage "C1039" @default.
- W2414278897 startingPage "C1030" @default.
- W2414278897 abstract "Endotoxin-mediated macrophage synthesis of nitric oxide (NO) is associated with immune effector function, intercellular communication, leukocyte adhesion, vascular integrity, and neurotransmission. However, little is known of the cellular receptor and signal transduction pathway by which endotoxin induces NO production. With the use of a model of ANA-1 murine macrophages, we stimulated NO production by incubation with increasing concentrations of endotoxin and 5% fetal calf serum. In selected instances, the anti-CD14 antibody, ED9, was added. Endotoxin-mediated NO synthesis was dependent on CD14 function and the presence of an additional serum factor. Endotoxin treatment increased plasma membrane GTPase activity and 35S-labeled guanosine 5'-O-(3-thiotriphosphate) ([35S]GTP gamma S) binding. Conversely, coincubation of cells with endotoxin and the heterotrimeric G protein inhibitors, suramin and guanosine 5'-O-(2-thiodiphosphate) trilithium salt, was associated with decreased NO synthesis, plasma membrane GTPase activity, and [35S]GTP gamma S binding. Blockade of CD14 or G protein function was associated with ablation of endotoxin-mediated inducible NO synthase (iNOS) protein expression, iNOS mRNA levels, and iNOS gene transcription, as determined by immunoblot, reverse transcriptase-polymerase chain reaction, and nuclear run-on analyses, respectively. These results indicate that endotoxin-mediated NO synthesis is a CD14-heterotrimeric G protein-dependent process." @default.
- W2414278897 created "2016-06-24" @default.
- W2414278897 creator A5016777555 @default.
- W2414278897 creator A5049080752 @default.
- W2414278897 creator A5061400435 @default.
- W2414278897 date "1997-09-01" @default.
- W2414278897 modified "2023-10-12" @default.
- W2414278897 title "CD14-dependent mechanism for endotoxin-mediated nitric oxide synthesis in murine macrophages" @default.
- W2414278897 cites W1481630279 @default.
- W2414278897 cites W1618819787 @default.
- W2414278897 cites W1775749144 @default.
- W2414278897 cites W1874295446 @default.
- W2414278897 cites W1975215292 @default.
- W2414278897 cites W2015994643 @default.
- W2414278897 cites W2055291298 @default.
- W2414278897 cites W2076118656 @default.
- W2414278897 cites W2148850683 @default.
- W2414278897 cites W2157502804 @default.
- W2414278897 doi "https://doi.org/10.1152/ajpcell.1997.273.3.c1030" @default.
- W2414278897 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9316424" @default.
- W2414278897 hasPublicationYear "1997" @default.
- W2414278897 type Work @default.
- W2414278897 sameAs 2414278897 @default.
- W2414278897 citedByCount "23" @default.
- W2414278897 crossrefType "journal-article" @default.
- W2414278897 hasAuthorship W2414278897A5016777555 @default.
- W2414278897 hasAuthorship W2414278897A5049080752 @default.
- W2414278897 hasAuthorship W2414278897A5061400435 @default.
- W2414278897 hasConcept C134018914 @default.
- W2414278897 hasConcept C139407321 @default.
- W2414278897 hasConcept C153911025 @default.
- W2414278897 hasConcept C170493617 @default.
- W2414278897 hasConcept C185592680 @default.
- W2414278897 hasConcept C2777995097 @default.
- W2414278897 hasConcept C2778513237 @default.
- W2414278897 hasConcept C49773422 @default.
- W2414278897 hasConcept C519581460 @default.
- W2414278897 hasConcept C55493867 @default.
- W2414278897 hasConcept C62478195 @default.
- W2414278897 hasConcept C80631254 @default.
- W2414278897 hasConcept C86803240 @default.
- W2414278897 hasConcept C95444343 @default.
- W2414278897 hasConceptScore W2414278897C134018914 @default.
- W2414278897 hasConceptScore W2414278897C139407321 @default.
- W2414278897 hasConceptScore W2414278897C153911025 @default.
- W2414278897 hasConceptScore W2414278897C170493617 @default.
- W2414278897 hasConceptScore W2414278897C185592680 @default.
- W2414278897 hasConceptScore W2414278897C2777995097 @default.
- W2414278897 hasConceptScore W2414278897C2778513237 @default.
- W2414278897 hasConceptScore W2414278897C49773422 @default.
- W2414278897 hasConceptScore W2414278897C519581460 @default.
- W2414278897 hasConceptScore W2414278897C55493867 @default.
- W2414278897 hasConceptScore W2414278897C62478195 @default.
- W2414278897 hasConceptScore W2414278897C80631254 @default.
- W2414278897 hasConceptScore W2414278897C86803240 @default.
- W2414278897 hasConceptScore W2414278897C95444343 @default.
- W2414278897 hasIssue "3" @default.
- W2414278897 hasLocation W24142788971 @default.
- W2414278897 hasLocation W24142788972 @default.
- W2414278897 hasOpenAccess W2414278897 @default.
- W2414278897 hasPrimaryLocation W24142788971 @default.
- W2414278897 hasRelatedWork W149771293 @default.
- W2414278897 hasRelatedWork W2005400238 @default.
- W2414278897 hasRelatedWork W2058388162 @default.
- W2414278897 hasRelatedWork W2096031452 @default.
- W2414278897 hasRelatedWork W215311856 @default.
- W2414278897 hasRelatedWork W2414278897 @default.
- W2414278897 hasRelatedWork W4220692437 @default.
- W2414278897 hasRelatedWork W4231116471 @default.
- W2414278897 hasRelatedWork W4242288745 @default.
- W2414278897 hasRelatedWork W2467371885 @default.
- W2414278897 hasVolume "273" @default.
- W2414278897 isParatext "false" @default.
- W2414278897 isRetracted "false" @default.
- W2414278897 magId "2414278897" @default.
- W2414278897 workType "article" @default.