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- W2414354673 abstract "OBJECTIVE To investigate the effect of high dose fluoride which ingested by female rats on morphologic change in rat offspring's bone and osteoblast, discuss the relation between the mechanism of fluorosis and cell cycle, cell apoptosis. METHODS In stock diets condition, Wistar female rats drank distilled water containing 0,50,100,150 mg/L NaF for 2 months, then they are mated with normal rats. The calvarium and osteoblast of offsprings were used to investigate the effects of fluoride on ultrastructure by LM and TEM. FCM was used to analysis cell cycle and apoptosis. RESULTS The Electron microscope revealed the number of microvilli of osteoblasts were overall decreased in rat offsprings with fluorosis. There was mitochondrial swelling and dilation of the rough endoplasmic reticulum (RER). The matrix of calvarium was hyperplasia and collagen was accumulated and turbulenced. The nuclear manifested the apoptosis character. NaF at 150 mg/ L increased the osteoblast number of S phase with relative decrease of cell number of G2/M phase, but did not change that in G0/G1 phase. The apoptosis percentage increased in this group. CONCLUSION Excessive fluoride can directly through the placental barrier, influence cell structure and cell cycle distribution of fluorosis rat offspring and render the cell cycle stagnant in S phase, induce apoptosis." @default.
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- W2414354673 date "2005-01-01" @default.
- W2414354673 modified "2023-09-24" @default.
- W2414354673 title "The effect of high dose fluoride on the rat offspring osteoblasts which ingested by female rats" @default.
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