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- W2414376778 abstract "This investigation was designed to explore the possible role of NADPH and reduced glutathione in insulin secretion and their relationship to thiol groups located in the β-cell membrane. We studied the effects of 2 µg/ml of methylene blue (an oxidant of NADPH), 0.1 mM diamide (an oxidant of GSH), and 2 mM tert -butyl hydroperoxide (a substrate of GSH peroxidase) on the release of insulin from isolated, perfused rat pancreas and pancreatic islets. p -Chloromercuribenzoate (a thiol reagent triggering insulin release supposedly by virtue of its action on superficial thiol groups in β-cell membranes) was used at 0.1 mM to initiate insulin secretion from isolated, perfused rat pancreas, while isolated islets were stimulated to release insulin with 3 mg/ml of glucose. Both phases of p -chloromercuribenzoate-triggered insulin release were significantly suppressed by methylene blue, diamide, and tert -butyl hydroperoxide. Similarly, these substances inhibited glucose-stimulated insulin release from isolated islets. These results suggest that stimulation of insulin release by p -chloromercuribenzoate and glucose depends on the content of NADPH and GSH in the islets. It is postulated that thiol groups in the β-cell membrane, which are thought to be related to insulin release, are kept in the reduced state via the following sequence of events:$See PDF for EquationACKNOWLEDGMENTS The authors wish to thank Miss I. Breuning and Mrs. I. Hagenloh for their skillful technical assistance. We are also indebted to Dr. O. Vitzthum of HAG AG for a generous supply of diamide, and to Professor Dr. A. Wendel for helpful advice." @default.
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- W2414376778 date "1977-07-01" @default.
- W2414376778 modified "2023-09-23" @default.
- W2414376778 title "Inhibition of p-chloromercuribenzoate- and glucose-induced insulin release in vitro by methylene blue, diamide, and tert-butyl hydroperoxide." @default.
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