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• W2414431549 abstract "Objective To establish a sprouting embryoid body model mimicking early embryonicvasculogenesis in human embryo. Methods Human embryonic stem were (hESCs) were cultured on themouse embryo fibloblasts and then were induced to differentiate to form three-dimensional EB. The hEBswere cultured in media containing various angiogenesis-related factors: vascular endothelial growth factor(VEGF), fibroblast growth factor (FGF), endostatin, angiostatin, and platelet factor (PF) -4 of differentconcentrations for 3 days to observe the sprouting of the hEBs. 3,3,3', 3'-tetramethylindo-carbocyanineperchlorate labeled acetylated low density lipoprotein (Dil-AcLDL) was added onto the hEBs foe 4 hImmunofluorescence assay was used to observe if Dil-AcLDL was absorbed and if CD31 was expressed so asto determine the existence of embryonic endothelial cells in the sprouting structures. The ideal culturingcondition was analyzed. Results The differentiated EBs formed sprouting structures in the collagen Ⅰmatrix containing VEGF and FGF. The sprouts among individual EBs were able to link to each other andform vascular network-like structures. In the presence of VEGF and FGF, the sprouts branching from theEBs assimilated DiI-AcLDL, expressed CD31 and formed a 3-dimensional cylindrical organization. Theconcentrations of growth factors ideally stimulating sprouting growth were 100 ng/ml of VEGF and 50 ng/mlof FGF. The networks among the EBs were abolished by the angiostatin, endostatin, and PF4. ConclusionThe sprouting from hEBs accumulates embryonic endothelial cells and the sprouting network-like structuresare indeed endothelial in nature. Inducing of sprouting EBs is an ideal model that mimics early embryonicvasculogenesis in humans.Key words: Embryonic stem cell, human;  Differentiation;  Embryo development; Vasculogenesis;  Embryo body" @default.
• W2414431549 created "2016-06-24" @default.
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• W2414431549 date "2008-10-14" @default.
• W2414431549 modified "2023-09-27" @default.
• W2414431549 title "Establishment of sprouting embryoid body model mimicking early embryonic vasculogenesis in human embryo" @default.
• W2414431549 doi "https://doi.org/10.3321/j.issn:0376-2491.2008.37.012" @default.
• W2414431549 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19080715" @default.
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