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• W2414441015 endingPage "9013" @default.
• W2414441015 startingPage "9001" @default.
• W2414441015 abstract "The class III ribonucleotide reductases (RNRs) are glycyl radical (G•) enzymes that provide the balanced pool of deoxynucleotides required for DNA synthesis and repair in many facultative and obligate anaerobic bacteria and archaea. Unlike the class I and II RNRs, where reducing equivalents for the reaction are delivered by a redoxin (thioredoxin, glutaredoxin, or NrdH) via a pair of conserved active site cysteines, the class III RNRs examined to date use formate as the reductant. Here, we report that reaction of the Escherichia coli class III RNR with CTP (substrate) and ATP (allosteric effector) in the absence of formate leads to loss of the G• concomitant with stoichiometric formation of a new radical species and a “trapped” cytidine derivative that can break down to cytosine. Addition of formate to the new species results in recovery of 80% of the G• and reduction of the cytidine derivative, proposed to be 3′-keto-deoxycytidine, to dCTP and a small amount of cytosine. The structure of the new radical has been identified by 9.5 and 140 GHz EPR spectroscopy on isotopically labeled varieties of the protein to be a thiosulfuranyl radical [RSSR2]•, composed of a cysteine thiyl radical stabilized by an interaction with a methionine residue. The presence of a stable radical species on the reaction pathway rationalizes the previously reported [3H]-(kcat/KM) isotope effect of 2.3 with [3H]-formate, requiring formate to exchange between the active site and solution during nucleotide reduction. Analogies with the disulfide anion radical proposed to provide the reducing equivalent to the 3′-keto-deoxycytidine intermediate by the class I and II RNRs provide further evidence for the involvement of thiyl radicals in the reductive half-reaction catalyzed by all RNRs." @default.
• W2414441015 created "2016-06-24" @default.
• W2414441015 creator A5000269609 @default.
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• W2414441015 creator A5074856177 @default.
• W2414441015 date "2014-06-17" @default.
• W2414441015 modified "2023-09-30" @default.
• W2414441015 title "A Chemically Competent Thiosulfuranyl Radical on the <i>Escherichia coli</i> Class III Ribonucleotide Reductase" @default.
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• W2414441015 cites W1513923893 @default.
• W2414441015 cites W1527837715 @default.
• W2414441015 cites W1538196126 @default.
• W2414441015 cites W1568700182 @default.
• W2414441015 cites W1605201659 @default.
• W2414441015 cites W1653081612 @default.
• W2414441015 cites W1918494586 @default.
• W2414441015 cites W1966521033 @default.
• W2414441015 cites W1967477066 @default.
• W2414441015 cites W1967899878 @default.
• W2414441015 cites W1968958940 @default.
• W2414441015 cites W1973844844 @default.
• W2414441015 cites W1974521432 @default.
• W2414441015 cites W1975967012 @default.
• W2414441015 cites W1976729393 @default.
• W2414441015 cites W1977560251 @default.
• W2414441015 cites W1979122292 @default.
• W2414441015 cites W1979798983 @default.
• W2414441015 cites W1983087517 @default.
• W2414441015 cites W1983901754 @default.
• W2414441015 cites W1986305567 @default.
• W2414441015 cites W1990434678 @default.
• W2414441015 cites W1991536506 @default.
• W2414441015 cites W1995966391 @default.
• W2414441015 cites W1996153116 @default.
• W2414441015 cites W1998165486 @default.
• W2414441015 cites W2001633114 @default.
• W2414441015 cites W2004460728 @default.
• W2414441015 cites W2005783000 @default.
• W2414441015 cites W2008028586 @default.
• W2414441015 cites W2012512658 @default.
• W2414441015 cites W2023913818 @default.
• W2414441015 cites W2025456320 @default.
• W2414441015 cites W2029667189 @default.
• W2414441015 cites W2030350346 @default.
• W2414441015 cites W2035067626 @default.
• W2414441015 cites W2042239319 @default.
• W2414441015 cites W2042446618 @default.
• W2414441015 cites W2043900297 @default.
• W2414441015 cites W2045645700 @default.
• W2414441015 cites W2047119353 @default.
• W2414441015 cites W2050088367 @default.
• W2414441015 cites W2053346654 @default.
• W2414441015 cites W2053954798 @default.
• W2414441015 cites W2055672280 @default.
• W2414441015 cites W2059824310 @default.
• W2414441015 cites W2068973845 @default.
• W2414441015 cites W2071291782 @default.
• W2414441015 cites W2072289725 @default.
• W2414441015 cites W2075330404 @default.
• W2414441015 cites W2084086913 @default.
• W2414441015 cites W2084734772 @default.
• W2414441015 cites W2085204013 @default.
• W2414441015 cites W2094326479 @default.
• W2414441015 cites W2094827467 @default.
• W2414441015 cites W2097198628 @default.
• W2414441015 cites W2101231282 @default.
• W2414441015 cites W2102620164 @default.
• W2414441015 cites W2105199418 @default.
• W2414441015 cites W2111967267 @default.
• W2414441015 cites W2113185442 @default.
• W2414441015 cites W2120901082 @default.
• W2414441015 cites W2143981217 @default.
• W2414441015 cites W2146051607 @default.
• W2414441015 cites W2150710339 @default.
• W2414441015 cites W2151620241 @default.
• W2414441015 cites W2155596226 @default.
• W2414441015 cites W2159285197 @default.
• W2414441015 cites W2163503178 @default.
• W2414441015 cites W2164318957 @default.
• W2414441015 cites W2170845296 @default.
• W2414441015 cites W2216826500 @default.
• W2414441015 cites W2319582671 @default.
• W2414441015 cites W2330997671 @default.
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• W2414441015 doi "https://doi.org/10.1021/ja5030194" @default.
• W2414441015 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4073831" @default.
• W2414441015 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24827372" @default.
• W2414441015 hasPublicationYear "2014" @default.
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