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- W2414442316 abstract "Measurement of the blood sugar is a frequent procedure in clinical laboratories but, from the standpoint of accuracy, perhaps one of the least satisfying to the clinical chemist. The fact that no known specific reaction is suitable for routine quantitative measurement of glucose necessitates the use of the nonspecific property of reduction. Since considerable amounts of noncarbohydrate material contribute to the reducing capacity of blood filtrates as they are frequently prepared (1, 2), there have been many attempts to increase the specificity of method. These attempts have logically taken two directions: (1) Investigation of deproteinizing agents which should remove from the blood filtrate most or all of the interfering substances (3, 4); (2) search for a chemical which will react only with glucose and be unaffected by other reducing materials present (5). The latter approach has resulted in an unusually simple blood sugar method which will be described. Anthrone has long been used as an analytical reagent for glycerol with which it forms a reddish-orange complex. Dreywood (6), in 1946, found that a highly specific color reaction was produced by anthrone in sulfuric acid with carbohydrate solutions and suggested it as a qualitative test for carbohydrate material. He examined 18 carbohydrates and found that all of them gave a positive test. They included cellulose, starch, dextrin, glucose, arabinose, gum arabic, ethyl cellulose, and cellulose acetate. Negative tests were obtained for a large group of noncarbohydrates, including a variety of organic acids, aldehydes. ketones, phenols, fats, proteins, and synthetic noncellulose resins. The only noncarbohydrate tested which gave the reaction was furfural, but its behavior was not typical since the green color was rapidly replaced by the formation of a brown precipitate. Dreywood found the anthrone reagent to be about 40 times more sensitive than iodine in the detection of starch. As little as 1 part of starch in 900,000 parts of water was reported to produce the characteristic color." @default.
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- W2414442316 date "1950-05-19" @default.
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- W2414442316 title "Rapid measurement of carbohydrate in blood." @default.
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