Matches in SemOpenAlex for { <https://semopenalex.org/work/W2414482810> ?p ?o ?g. }
Showing items 1 to 90 of
90
with 100 items per page.
- W2414482810 endingPage "19" @default.
- W2414482810 startingPage "109" @default.
- W2414482810 abstract "Cardiac sarcolemmal (SL) phospholipase C (PLC) is a key enzyme in the signal transduction of several cardiac receptors. Thus, the earlier described Ca(2+)-stimulated SL PLC activity may represent variously coupled enzymes. The present study was undertaken to delineate the alpha 1-adrenoceptor/G protein-stimulated PLC activity in purified cardiac SL vesicles. Although certain detergents and membrane pore formers enhanced SL PLC activity, measured as formation of 3H-inositol 1,4,5-trisphosphate [Ins(1,4,5)P3] from 3H-phosphatidylinositol 4,5-bisphosphate, alpha 1-adrenoceptor stimulated activity was not observed. When SL vesicles were preincubated (0-4 degrees C) with substrate in detergent-free buffer, subsequent incubation (37 degrees C; in mM: 100 NaCl, 2 EGTA, 1.8 CaCl2, 10 LiCl) resulted in a time-dependent production of 3H-Ins(1,4,5)P3, that was increased in the presence of 100 microM GTP gamma S. GTP gamma S stimulation of SL PLC activity required the presence of Mg2+ and Ca2+, but was lost at (sub)millimolar concentrations of these bivalent cations. Mg2+ (0.01-10 mM) promoted a 2,3-diphosphoglycerate-insensitive phosphatase activity. GTP gamma S enhanced the sensitivity of SL PLC to Ca2+, but did not increase the maximum Ca2+ (0.1-1 mM) stimulated SL PLC activity. At 5 microM Ca2+, GTP gamma S induced a concentration-dependent rise in inositol phosphates production, which was further elevated by the alpha 1-agonist, phenylephrine (PhE). The PhE-effect was inhibited by the alpha 1-antagonist prazosin, but not by the beta-antagonist atenolol. These results show that the components necessary for the alpha 1-adrenoceptor transmembrane signal are associated with the SL membrane and can be functionally coupled." @default.
- W2414482810 created "2016-06-24" @default.
- W2414482810 creator A5011896624 @default.
- W2414482810 creator A5049484003 @default.
- W2414482810 creator A5065861882 @default.
- W2414482810 date "1994-01-01" @default.
- W2414482810 modified "2023-09-23" @default.
- W2414482810 title "Alpha 1-adrenergic stimulation of phospholipase C activity in purified cardiac sarcolemmal membranes." @default.
- W2414482810 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7950979" @default.
- W2414482810 hasPublicationYear "1994" @default.
- W2414482810 type Work @default.
- W2414482810 sameAs 2414482810 @default.
- W2414482810 citedByCount "0" @default.
- W2414482810 crossrefType "journal-article" @default.
- W2414482810 hasAuthorship W2414482810A5011896624 @default.
- W2414482810 hasAuthorship W2414482810A5049484003 @default.
- W2414482810 hasAuthorship W2414482810A5065861882 @default.
- W2414482810 hasConcept C118716 @default.
- W2414482810 hasConcept C126322002 @default.
- W2414482810 hasConcept C130316041 @default.
- W2414482810 hasConcept C134018914 @default.
- W2414482810 hasConcept C170493617 @default.
- W2414482810 hasConcept C178790620 @default.
- W2414482810 hasConcept C181199279 @default.
- W2414482810 hasConcept C185592680 @default.
- W2414482810 hasConcept C199096232 @default.
- W2414482810 hasConcept C24998067 @default.
- W2414482810 hasConcept C2776885963 @default.
- W2414482810 hasConcept C2777427919 @default.
- W2414482810 hasConcept C2777658833 @default.
- W2414482810 hasConcept C2777952589 @default.
- W2414482810 hasConcept C2778597717 @default.
- W2414482810 hasConcept C2778938600 @default.
- W2414482810 hasConcept C2780610907 @default.
- W2414482810 hasConcept C2780648677 @default.
- W2414482810 hasConcept C41625074 @default.
- W2414482810 hasConcept C519063684 @default.
- W2414482810 hasConcept C55493867 @default.
- W2414482810 hasConcept C62478195 @default.
- W2414482810 hasConcept C71924100 @default.
- W2414482810 hasConcept C80631254 @default.
- W2414482810 hasConcept C84393581 @default.
- W2414482810 hasConcept C86803240 @default.
- W2414482810 hasConceptScore W2414482810C118716 @default.
- W2414482810 hasConceptScore W2414482810C126322002 @default.
- W2414482810 hasConceptScore W2414482810C130316041 @default.
- W2414482810 hasConceptScore W2414482810C134018914 @default.
- W2414482810 hasConceptScore W2414482810C170493617 @default.
- W2414482810 hasConceptScore W2414482810C178790620 @default.
- W2414482810 hasConceptScore W2414482810C181199279 @default.
- W2414482810 hasConceptScore W2414482810C185592680 @default.
- W2414482810 hasConceptScore W2414482810C199096232 @default.
- W2414482810 hasConceptScore W2414482810C24998067 @default.
- W2414482810 hasConceptScore W2414482810C2776885963 @default.
- W2414482810 hasConceptScore W2414482810C2777427919 @default.
- W2414482810 hasConceptScore W2414482810C2777658833 @default.
- W2414482810 hasConceptScore W2414482810C2777952589 @default.
- W2414482810 hasConceptScore W2414482810C2778597717 @default.
- W2414482810 hasConceptScore W2414482810C2778938600 @default.
- W2414482810 hasConceptScore W2414482810C2780610907 @default.
- W2414482810 hasConceptScore W2414482810C2780648677 @default.
- W2414482810 hasConceptScore W2414482810C41625074 @default.
- W2414482810 hasConceptScore W2414482810C519063684 @default.
- W2414482810 hasConceptScore W2414482810C55493867 @default.
- W2414482810 hasConceptScore W2414482810C62478195 @default.
- W2414482810 hasConceptScore W2414482810C71924100 @default.
- W2414482810 hasConceptScore W2414482810C80631254 @default.
- W2414482810 hasConceptScore W2414482810C84393581 @default.
- W2414482810 hasConceptScore W2414482810C86803240 @default.
- W2414482810 hasIssue "2" @default.
- W2414482810 hasLocation W24144828101 @default.
- W2414482810 hasOpenAccess W2414482810 @default.
- W2414482810 hasPrimaryLocation W24144828101 @default.
- W2414482810 hasRelatedWork W1562774015 @default.
- W2414482810 hasRelatedWork W2000792791 @default.
- W2414482810 hasRelatedWork W2010332437 @default.
- W2414482810 hasRelatedWork W2064318241 @default.
- W2414482810 hasRelatedWork W2081249121 @default.
- W2414482810 hasRelatedWork W2139242677 @default.
- W2414482810 hasRelatedWork W2411203475 @default.
- W2414482810 hasRelatedWork W2414482810 @default.
- W2414482810 hasRelatedWork W2415294454 @default.
- W2414482810 hasRelatedWork W79426895 @default.
- W2414482810 hasVolume "4" @default.
- W2414482810 isParatext "false" @default.
- W2414482810 isRetracted "false" @default.
- W2414482810 magId "2414482810" @default.
- W2414482810 workType "article" @default.