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- W2414491725 abstract "The enzyme-linked immunosorbent assay (ELISA) was developed by Engvall & Perlmann. Recently Voller & Bidwell introduced a micro modification of this assay for detection of viral antibodies.It is known that many nonspecific factors may affect the outcome of this test. These factors must be eliminated for application to the routine serological test.In this study micro-ELISA is adapted for varicella zoster, measles and rubella virus by elimination of nonspecific reaction. The procedures as follows enable us to detect each antibody.1. The addition of sodium azide to the enzyme-conjugated solution and the adsorption anti -human embryo lung antibodies from sera employed are effective factors in the detection of ELISA antib odies to varicella zoster virus.2. And in the detection of antibodies to measles and rubella virus, the addition of sodium azids and coating sensitized wells with I% BSA are effective in the success of the ELISA procedure." @default.
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- W2414491725 date "1981-01-01" @default.
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- W2414491725 title "The Elimination of Nonspecific Reaction in ELISA" @default.
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- W2414491725 doi "https://doi.org/10.11150/kansenshogakuzasshi1970.55.143" @default.
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