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- W2414496677 abstract "This chapter describes three different strategies of receptor mutagenesis with their advantages, disadvantages, and limitations. Oligonucleotide-directed mutagenesis using either the Altered Sites® II in vitro mutagenesis system or the GeneTailor® site-directed mutagenesis system can generate base substitutions/deletions/insertions that yield single/multiple amino acid substitutions/deletions/insertions and/or N- or C-terminal truncations in GPCRs. Polymerase chain reaction-based mutagenesis strategies allow substitutions/deletions/insertions of larger domains within GPCRs, creating truncated receptors or receptor chimeras.In addition, some guidelines are given and examples are provided to facilitate design and interpretation of mutational experiments." @default.
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- W2414496677 date "2004-02-09" @default.
- W2414496677 modified "2023-10-06" @default.
- W2414496677 title "Receptor Mutagenesis Strategies for Examination of Structure–Function Relationships" @default.
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- W2414496677 doi "https://doi.org/10.1385/1-59259-754-8:307" @default.
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