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• W2414690377 startingPage "217" @default.
• W2414690377 abstract "TFIIB-like general transcription factors are required for transcription initiation by all eukaryotic and archaeal RNA polymerases (RNAPs). TFIIB facilitates both recruitment and post-recruitment steps of initiation; in particular, TFIIB stimulates abortive initiation. X-ray crystallography of TFIIB-RNAP II complexes shows that the TFIIB linker region penetrates the RNAP active center, yet the impact of this arrangement on RNAP activity and underlying mechanisms remains elusive. Promoter-independent abortive initiation assays exploit the intrinsic ability of RNAP enzymes to initiate transcription from nicked DNA templates and record the formation of the first phosphodiester bonds. These assays can be used to measure the effect of transcription factors such as TFIIB and RNAP mutations on abortive transcription." @default.
• W2414690377 created "2016-06-24" @default.
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• W2414690377 creator A5043620755 @default.
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• W2414690377 date "2013-01-01" @default.
• W2414690377 modified "2023-09-26" @default.
• W2414690377 title "Promoter Independent Abortive Transcription Assays Unravel Functional Interactions Between TFIIB and RNA Polymerase" @default.
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• W2414690377 doi "https://doi.org/10.1007/978-1-62703-284-1_17" @default.
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• W2414690377 hasPublicationYear "2013" @default.
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