Matches in SemOpenAlex for { <https://semopenalex.org/work/W2414741589> ?p ?o ?g. }
Showing items 1 to 95 of
95
with 100 items per page.
- W2414741589 endingPage "C714" @default.
- W2414741589 startingPage "C708" @default.
- W2414741589 abstract "Adenosine formation by the heart is known to critically depend on the ratio of oxygen supply to oxygen demand, but the sensitivity of cardiomyocytes to defined changes in PO2 is not known. Isolated metabolically stable rat cardiomyocytes were incubated up to 45 min at constant PO2 values ranging from 0.1 to 100 mmHg using a feedback-controlled incubation system (oxystat system). Changes of the free intracellular adenosine concentration were measured after trapping of adenosine by cytosolic S-adenosylhomocysteine (SAH) hydrolase in the presence of 200 microM L-homocysteine thiolactone. Rate of SAH formation was constant at a PO2 between 3 and 100 mmHg and gradually increased at PO2 less than 3 mmHg. Cellular ATP decreased only at PO2 less than 1 mmHg, and this was accompanied by a decline of oxygen consumption. Treatment of cells with 5.5 mM deoxyglucose and 4 micrograms/ml oligomycin increased SAH formation 60-fold and was associated with elevated intra- and to a lesser extent extracellular adenosine levels. Inhibition of nucleoside transport with 20 microM S-(p-nitrobenzyl)-6-thioinosine steepened the transmembrane adenosine gradient. Our findings suggest that the cardiomyocyte responds to metabolic poisoning and oxygen deprivation with an enhanced formation of adenosine. This adenosine is mainly formed intracellularly and reaches the extracellular space by diffusion. Threshold for adenosine formation is as low as 3 mmHg." @default.
- W2414741589 created "2016-06-24" @default.
- W2414741589 creator A5040387308 @default.
- W2414741589 creator A5057582462 @default.
- W2414741589 creator A5073363845 @default.
- W2414741589 creator A5077975434 @default.
- W2414741589 date "1991-04-01" @default.
- W2414741589 modified "2023-09-24" @default.
- W2414741589 title "Oxygen partial pressure and free intracellular adenosine of isolated cardiomyocytes" @default.
- W2414741589 cites W1504836130 @default.
- W2414741589 cites W1527962461 @default.
- W2414741589 cites W1775749144 @default.
- W2414741589 cites W1972726726 @default.
- W2414741589 cites W1996661902 @default.
- W2414741589 cites W1997192988 @default.
- W2414741589 cites W2028479043 @default.
- W2414741589 cites W2029054073 @default.
- W2414741589 cites W2039627554 @default.
- W2414741589 cites W2041393456 @default.
- W2414741589 cites W2045978620 @default.
- W2414741589 cites W2046451463 @default.
- W2414741589 cites W2046943389 @default.
- W2414741589 cites W2059032559 @default.
- W2414741589 cites W2067057440 @default.
- W2414741589 cites W2073199636 @default.
- W2414741589 cites W2077816025 @default.
- W2414741589 cites W2095656943 @default.
- W2414741589 cites W2096458937 @default.
- W2414741589 cites W2100770709 @default.
- W2414741589 cites W2302589454 @default.
- W2414741589 cites W2398205385 @default.
- W2414741589 doi "https://doi.org/10.1152/ajpcell.1991.260.4.c708" @default.
- W2414741589 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2018108" @default.
- W2414741589 hasPublicationYear "1991" @default.
- W2414741589 type Work @default.
- W2414741589 sameAs 2414741589 @default.
- W2414741589 citedByCount "46" @default.
- W2414741589 countsByYear W24147415892014 @default.
- W2414741589 countsByYear W24147415892015 @default.
- W2414741589 countsByYear W24147415892017 @default.
- W2414741589 crossrefType "journal-article" @default.
- W2414741589 hasAuthorship W2414741589A5040387308 @default.
- W2414741589 hasAuthorship W2414741589A5057582462 @default.
- W2414741589 hasAuthorship W2414741589A5073363845 @default.
- W2414741589 hasAuthorship W2414741589A5077975434 @default.
- W2414741589 hasConcept C12554922 @default.
- W2414741589 hasConcept C126322002 @default.
- W2414741589 hasConcept C178790620 @default.
- W2414741589 hasConcept C181199279 @default.
- W2414741589 hasConcept C185592680 @default.
- W2414741589 hasConcept C23265538 @default.
- W2414741589 hasConcept C2776991684 @default.
- W2414741589 hasConcept C2779134199 @default.
- W2414741589 hasConcept C28406088 @default.
- W2414741589 hasConcept C540031477 @default.
- W2414741589 hasConcept C55493867 @default.
- W2414741589 hasConcept C71924100 @default.
- W2414741589 hasConcept C79879829 @default.
- W2414741589 hasConcept C86803240 @default.
- W2414741589 hasConceptScore W2414741589C12554922 @default.
- W2414741589 hasConceptScore W2414741589C126322002 @default.
- W2414741589 hasConceptScore W2414741589C178790620 @default.
- W2414741589 hasConceptScore W2414741589C181199279 @default.
- W2414741589 hasConceptScore W2414741589C185592680 @default.
- W2414741589 hasConceptScore W2414741589C23265538 @default.
- W2414741589 hasConceptScore W2414741589C2776991684 @default.
- W2414741589 hasConceptScore W2414741589C2779134199 @default.
- W2414741589 hasConceptScore W2414741589C28406088 @default.
- W2414741589 hasConceptScore W2414741589C540031477 @default.
- W2414741589 hasConceptScore W2414741589C55493867 @default.
- W2414741589 hasConceptScore W2414741589C71924100 @default.
- W2414741589 hasConceptScore W2414741589C79879829 @default.
- W2414741589 hasConceptScore W2414741589C86803240 @default.
- W2414741589 hasIssue "4" @default.
- W2414741589 hasLocation W24147415891 @default.
- W2414741589 hasLocation W24147415892 @default.
- W2414741589 hasOpenAccess W2414741589 @default.
- W2414741589 hasPrimaryLocation W24147415891 @default.
- W2414741589 hasRelatedWork W101867907 @default.
- W2414741589 hasRelatedWork W13701512 @default.
- W2414741589 hasRelatedWork W2014034588 @default.
- W2414741589 hasRelatedWork W2023082027 @default.
- W2414741589 hasRelatedWork W2030915532 @default.
- W2414741589 hasRelatedWork W2085920417 @default.
- W2414741589 hasRelatedWork W2103030499 @default.
- W2414741589 hasRelatedWork W2111870424 @default.
- W2414741589 hasRelatedWork W2124724781 @default.
- W2414741589 hasRelatedWork W2408647218 @default.
- W2414741589 hasVolume "260" @default.
- W2414741589 isParatext "false" @default.
- W2414741589 isRetracted "false" @default.
- W2414741589 magId "2414741589" @default.
- W2414741589 workType "article" @default.