Matches in SemOpenAlex for { <https://semopenalex.org/work/W2414969331> ?p ?o ?g. }
Showing items 1 to 93 of
93
with 100 items per page.
- W2414969331 endingPage "227" @default.
- W2414969331 startingPage "222" @default.
- W2414969331 abstract "ObjectiveTo evaluate the effects of artemisinin on proliferation, cell cycle and apoptosis of gallbladder cancer cells.MethodsGallbladder carcinoma cell lines(GBC-SD and NOZ)were cultured in vitro. The effects of artemisinin in different concentration on proliferation of the two cell lines in vitro were examined using MTT assay. The cell cycle distribution of GBC-SD and NOZ cells 24 h after treatments with artemisinin(20 μmol/L) were examined using flow cytometry. The apoptosis of GBC-SD and NOZ cells 24 h after treatments with artemisinin (20 μmol/L) were examined using Annexin V/PI staining.The expressions of p-ERK1/2, CDK4, cyclin D1, p16, cytochrome C and caspase-3 were examined by Western blot assay. t-test and one way ANOVA were used to evaluate the differences between two groups and more than two groups, respectively.ResultsThe cell proliferation was significantly inhibited by artemisinin, the IC50 of artemisinin against GBC-SD and NOZ cells were 14.05 μmol/L and 12.42 μmol/L, respectively.Artemisinin induced cycle arrest, and G1 population of GBC-SD and NOZ cells increased to 74.60% and 78.86%. Cell apoptosis and apoptotic population of GBC-SD and NOZ cells were increased to 15.67% and 16.51% after dealt with artemisinin, respectively. In addition, expression of p16 was increased, and expressions of p-ERK1/2, CDK4 and cyclin D1 were down-regulated by artemisinin(all P<0.05). Cytochrome C was released from mitochondria to cytoplasm leading to the activation of caspase-3 and PARP after dealt with artemisinin(P<0.05).ConclusionThe inhibition effect of artemisinin on the proliferation gallbladder cancer cells is accompanied by down-regulation of ERK1/2 signaling pathway, G1 phase arrest and triggering caspase-3-mediate apoptosis.Key words: Gallbladder neoplasms; Artemisinins; Apoptosis" @default.
- W2414969331 created "2016-06-24" @default.
- W2414969331 creator A5015540778 @default.
- W2414969331 creator A5041525354 @default.
- W2414969331 creator A5042536909 @default.
- W2414969331 creator A5046138476 @default.
- W2414969331 creator A5054458578 @default.
- W2414969331 creator A5074166993 @default.
- W2414969331 creator A5086049083 @default.
- W2414969331 date "2016-03-01" @default.
- W2414969331 modified "2023-09-23" @default.
- W2414969331 title "Artemisinin inhibits proliferation of gallbladder cancer cell lines through triggering cell cycle arrest and apoptosis" @default.
- W2414969331 doi "https://doi.org/10.3760/cma.j.issn.0529-5815.2016.03.014" @default.
- W2414969331 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26932893" @default.
- W2414969331 hasPublicationYear "2016" @default.
- W2414969331 type Work @default.
- W2414969331 sameAs 2414969331 @default.
- W2414969331 citedByCount "0" @default.
- W2414969331 crossrefType "journal-article" @default.
- W2414969331 hasAuthorship W2414969331A5015540778 @default.
- W2414969331 hasAuthorship W2414969331A5041525354 @default.
- W2414969331 hasAuthorship W2414969331A5042536909 @default.
- W2414969331 hasAuthorship W2414969331A5046138476 @default.
- W2414969331 hasAuthorship W2414969331A5054458578 @default.
- W2414969331 hasAuthorship W2414969331A5074166993 @default.
- W2414969331 hasAuthorship W2414969331A5086049083 @default.
- W2414969331 hasConcept C105696609 @default.
- W2414969331 hasConcept C153911025 @default.
- W2414969331 hasConcept C190283241 @default.
- W2414969331 hasConcept C199835354 @default.
- W2414969331 hasConcept C203014093 @default.
- W2414969331 hasConcept C2776120307 @default.
- W2414969331 hasConcept C2778048844 @default.
- W2414969331 hasConcept C2778371730 @default.
- W2414969331 hasConcept C2780783641 @default.
- W2414969331 hasConcept C2908647359 @default.
- W2414969331 hasConcept C29537977 @default.
- W2414969331 hasConcept C55493867 @default.
- W2414969331 hasConcept C556039675 @default.
- W2414969331 hasConcept C62112901 @default.
- W2414969331 hasConcept C71924100 @default.
- W2414969331 hasConcept C86803240 @default.
- W2414969331 hasConcept C98274493 @default.
- W2414969331 hasConcept C99454951 @default.
- W2414969331 hasConceptScore W2414969331C105696609 @default.
- W2414969331 hasConceptScore W2414969331C153911025 @default.
- W2414969331 hasConceptScore W2414969331C190283241 @default.
- W2414969331 hasConceptScore W2414969331C199835354 @default.
- W2414969331 hasConceptScore W2414969331C203014093 @default.
- W2414969331 hasConceptScore W2414969331C2776120307 @default.
- W2414969331 hasConceptScore W2414969331C2778048844 @default.
- W2414969331 hasConceptScore W2414969331C2778371730 @default.
- W2414969331 hasConceptScore W2414969331C2780783641 @default.
- W2414969331 hasConceptScore W2414969331C2908647359 @default.
- W2414969331 hasConceptScore W2414969331C29537977 @default.
- W2414969331 hasConceptScore W2414969331C55493867 @default.
- W2414969331 hasConceptScore W2414969331C556039675 @default.
- W2414969331 hasConceptScore W2414969331C62112901 @default.
- W2414969331 hasConceptScore W2414969331C71924100 @default.
- W2414969331 hasConceptScore W2414969331C86803240 @default.
- W2414969331 hasConceptScore W2414969331C98274493 @default.
- W2414969331 hasConceptScore W2414969331C99454951 @default.
- W2414969331 hasIssue "3" @default.
- W2414969331 hasLocation W24149693311 @default.
- W2414969331 hasOpenAccess W2414969331 @default.
- W2414969331 hasPrimaryLocation W24149693311 @default.
- W2414969331 hasRelatedWork W1593583054 @default.
- W2414969331 hasRelatedWork W2009358451 @default.
- W2414969331 hasRelatedWork W2046569868 @default.
- W2414969331 hasRelatedWork W2053359933 @default.
- W2414969331 hasRelatedWork W2074567041 @default.
- W2414969331 hasRelatedWork W2075576602 @default.
- W2414969331 hasRelatedWork W2104462720 @default.
- W2414969331 hasRelatedWork W2263513337 @default.
- W2414969331 hasRelatedWork W2345600927 @default.
- W2414969331 hasRelatedWork W2352026222 @default.
- W2414969331 hasRelatedWork W2379600208 @default.
- W2414969331 hasRelatedWork W2394835454 @default.
- W2414969331 hasRelatedWork W2521714063 @default.
- W2414969331 hasRelatedWork W2778503620 @default.
- W2414969331 hasRelatedWork W2981184832 @default.
- W2414969331 hasRelatedWork W2999162932 @default.
- W2414969331 hasRelatedWork W3029906927 @default.
- W2414969331 hasRelatedWork W3034018946 @default.
- W2414969331 hasRelatedWork W3127463016 @default.
- W2414969331 hasRelatedWork W3173304332 @default.
- W2414969331 hasVolume "54" @default.
- W2414969331 isParatext "false" @default.
- W2414969331 isRetracted "false" @default.
- W2414969331 magId "2414969331" @default.
- W2414969331 workType "article" @default.