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• W2414969680 abstract "ABSTRACT RNA editing sites in plant mitochondria and plastids are addressed by pentatricopeptide repeat (PPR) pro-teins with E or E and DYW domains, which recognize a specific nucleotide motif upstream of the editednucleotide. In addition, some sites require MORF proteins for efficient RNA editing. Here, we assign thenovel E domain-containing PPR protein, MEF13, as being required for editing at eight sites in Arabidopsisthaliana. A SNP in ecotype C24 altering the editing level at only one of the eight target sites was located bygenomic mapping. An EMS mutant allele of the gene for MEF13 was identified in a SNaPshot screen of amutated plant population. At all eight target sites of MEF13, editing levels are reduced in both morf3 andmorf8 mutants, but at only one site in morf1 mutants, suggesting that specific MEF13–MORF interactionsare required. Yeast two-hybrid analyses detect solid connections of MEF13 with MORF1 and weak contactwith MORF3 proteins. Yeast three-hybrid (Y3H) analysis shows that the presence of MORF8 enhances theconnection between MEF13 and MORF3, suggesting that a MORF3–MORF8 heteromer may form stably ortransiently to establish interaction with MEF13.Key words: RNA editing, plant mitochondria, PPR protein, MEF13, MEF–MORF interactionGlass F., Ha¨rtel B., ZehrmannA.,VerbitskiyD.,and TakenakaM.(2015).MEF13RequiresMORF3 and MORF8for RNA Editing at Eight Targets in Mitochondrial mRNAs in Arabidopsis thaliana. Mol. Plant. 8, 1466–1477." @default.
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• W2414969680 date "2015-01-01" @default.
• W2414969680 modified "2023-09-27" @default.
• W2414969680 title "MEF13 Requires MORF3 and MORF8 for RNA EditingatEightTargetsinMitochondrialmRNAsin" @default.
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