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• W2415175854 abstract "ABSTRACT Earlier studies have established that the average speed of a replication fork is two to three times slower in early S-phase than in late S-phase and that the intracellular 2′-deoxyribonucleoside 5′-triphosphate pools grow during S-phase. In this study, the effect of the exogenous 2′-deoxyribonucleoside 5′-triphosphate (dNTP) supply on the average replication speed in a synchronised population of human HeLa cells was tested. The speed of replication fork movement was measured on extended DNA fibers labelled with 2′-deoxythymidine analogues 5-chloro-2′-deoxyuridine and 5-iodo-2′-deoxyuridine. We show that the introduction of exogenous dNTPs accelerates the replication process at the beginning of DNA synthesis only. In late S-phase, the administration of additional dNTPs has no effect on the speed of replication forks. The availability of 2′-deoxynucleotides seems to be a rate-limiting factor for DNA replication during early S-phase." @default.
• W2415175854 created "2016-06-24" @default.
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• W2415175854 date "2001-02-15" @default.
• W2415175854 modified "2023-10-15" @default.
• W2415175854 title "The supply of exogenous deoxyribonucleotides accelerates the speed of the replication fork in early S-phase" @default.
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• W2415175854 doi "https://doi.org/10.1242/jcs.114.4.747" @default.
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