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- W2415211253 abstract "A method is described for generating conditional lethal mutations in essential genes in Escherichia coli. In this procedure, amber stop codons are introduced as tagalong mutations in the flanking DNA of a downstream antibiotic-resistance marker by lambda Red recombination. The marker is removed by expression of I-SceI homing endonuclease, leaving a markerless mutation. The mutants then depend upon expression of a suppressor transfer RNA (tRNA) for survival, which is expressed under control of the arabinose promoter on a high-copy-number plasmid." @default.
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- W2415211253 date "2008-01-01" @default.
- W2415211253 modified "2023-09-27" @default.
- W2415211253 title "Introduction of Conditional Lethal Amber Mutations in Escherichia coli" @default.
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- W2415211253 doi "https://doi.org/10.1007/978-1-59745-321-9_21" @default.
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