Matches in SemOpenAlex for { <https://semopenalex.org/work/W2415280474> ?p ?o ?g. }
- W2415280474 endingPage "203" @default.
- W2415280474 startingPage "197" @default.
- W2415280474 abstract "Adequate intracellular energy transfer is crucial for proper cardiac function. In energy starved failing hearts, partial restoration of energy transfer can rescue mechanical performance. There are two types of diffusion obstacles that interfere with energy transfer from mitochondria to ATPases: mitochondrial outer membrane (MOM) with voltage-dependent anion channel (VDAC) permeable to small hydrophilic molecules and cytoplasmatic diffusion barriers grouping ATP-producers and -consumers. So far, there is no method developed to clearly distinguish the contributions of cytoplasmatic barriers and MOM to the overall diffusion restriction. Furthermore, the number of open VDACs in vivo remains unknown. The aim of this work was to establish the partitioning of intracellular diffusion obstacles in cardiomyocytes. We studied the response of mitochondrial oxidative phosphorylation of permeabilized rat cardiomyocytes to changes in extracellular ADP by recording 3D image stacks of NADH autofluorescence. Using cell-specific mathematical models, we determined the permeability of MOM and cytoplasmatic barriers. We found that only ~2% of VDACs are accessible to cytosolic ADP and cytoplasmatic diffusion barriers reduce the apparent diffusion coefficient by 6-10×. In cardiomyocytes, diffusion barriers in the cytoplasm and by the MOM restrict ADP/ATP diffusion to similar extents suggesting a major role of both barriers in energy transfer and other intracellular processes." @default.
- W2415280474 created "2016-06-24" @default.
- W2415280474 creator A5029224152 @default.
- W2415280474 creator A5051733624 @default.
- W2415280474 creator A5074075541 @default.
- W2415280474 creator A5081064992 @default.
- W2415280474 creator A5085655849 @default.
- W2415280474 creator A5091141927 @default.
- W2415280474 date "2016-08-01" @default.
- W2415280474 modified "2023-09-24" @default.
- W2415280474 title "Restricted ADP movement in cardiomyocytes: Cytosolic diffusion obstacles are complemented with a small number of open mitochondrial voltage-dependent anion channels" @default.
- W2415280474 cites W1481471836 @default.
- W2415280474 cites W1558050921 @default.
- W2415280474 cites W1766184155 @default.
- W2415280474 cites W1941608595 @default.
- W2415280474 cites W1973484140 @default.
- W2415280474 cites W1982059443 @default.
- W2415280474 cites W1986480651 @default.
- W2415280474 cites W1987031977 @default.
- W2415280474 cites W1987888574 @default.
- W2415280474 cites W1991931009 @default.
- W2415280474 cites W1997759034 @default.
- W2415280474 cites W2003834810 @default.
- W2415280474 cites W2007546912 @default.
- W2415280474 cites W2007552499 @default.
- W2415280474 cites W2010553753 @default.
- W2415280474 cites W2015859860 @default.
- W2415280474 cites W2024093825 @default.
- W2415280474 cites W2024506048 @default.
- W2415280474 cites W2027605021 @default.
- W2415280474 cites W2028436089 @default.
- W2415280474 cites W2031834615 @default.
- W2415280474 cites W2040171040 @default.
- W2415280474 cites W2048408738 @default.
- W2415280474 cites W2048852087 @default.
- W2415280474 cites W2052733759 @default.
- W2415280474 cites W2055971178 @default.
- W2415280474 cites W2060801398 @default.
- W2415280474 cites W2062967090 @default.
- W2415280474 cites W2063084645 @default.
- W2415280474 cites W2066987581 @default.
- W2415280474 cites W2069342809 @default.
- W2415280474 cites W2069962416 @default.
- W2415280474 cites W2072466390 @default.
- W2415280474 cites W2072528174 @default.
- W2415280474 cites W2081586404 @default.
- W2415280474 cites W2085754322 @default.
- W2415280474 cites W2092301915 @default.
- W2415280474 cites W2098431001 @default.
- W2415280474 cites W2107141336 @default.
- W2415280474 cites W2112720988 @default.
- W2415280474 cites W2123827864 @default.
- W2415280474 cites W2125525757 @default.
- W2415280474 cites W2125595015 @default.
- W2415280474 cites W2136438732 @default.
- W2415280474 cites W2140258011 @default.
- W2415280474 cites W2143753516 @default.
- W2415280474 cites W2147847000 @default.
- W2415280474 cites W2156381819 @default.
- W2415280474 cites W2163476271 @default.
- W2415280474 cites W2175859329 @default.
- W2415280474 cites W2281889878 @default.
- W2415280474 cites W4243622324 @default.
- W2415280474 cites W4250737642 @default.
- W2415280474 doi "https://doi.org/10.1016/j.yjmcc.2016.04.012" @default.
- W2415280474 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27261153" @default.
- W2415280474 hasPublicationYear "2016" @default.
- W2415280474 type Work @default.
- W2415280474 sameAs 2415280474 @default.
- W2415280474 citedByCount "26" @default.
- W2415280474 countsByYear W24152804742016 @default.
- W2415280474 countsByYear W24152804742017 @default.
- W2415280474 countsByYear W24152804742018 @default.
- W2415280474 countsByYear W24152804742019 @default.
- W2415280474 countsByYear W24152804742020 @default.
- W2415280474 countsByYear W24152804742021 @default.
- W2415280474 countsByYear W24152804742022 @default.
- W2415280474 countsByYear W24152804742023 @default.
- W2415280474 crossrefType "journal-article" @default.
- W2415280474 hasAuthorship W2415280474A5029224152 @default.
- W2415280474 hasAuthorship W2415280474A5051733624 @default.
- W2415280474 hasAuthorship W2415280474A5074075541 @default.
- W2415280474 hasAuthorship W2415280474A5081064992 @default.
- W2415280474 hasAuthorship W2415280474A5085655849 @default.
- W2415280474 hasAuthorship W2415280474A5091141927 @default.
- W2415280474 hasConcept C104317684 @default.
- W2415280474 hasConcept C112243037 @default.
- W2415280474 hasConcept C121332964 @default.
- W2415280474 hasConcept C12554922 @default.
- W2415280474 hasConcept C146587185 @default.
- W2415280474 hasConcept C181199279 @default.
- W2415280474 hasConcept C185592680 @default.
- W2415280474 hasConcept C190062978 @default.
- W2415280474 hasConcept C200784783 @default.
- W2415280474 hasConcept C28406088 @default.
- W2415280474 hasConcept C28859421 @default.
- W2415280474 hasConcept C547475151 @default.
- W2415280474 hasConcept C55493867 @default.