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- W2415393265 endingPage "300" @default.
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- W2415393265 abstract "The rapid embryonic development and high fecundity of zebrafish contribute to the great advantages of this model for the study of developmental genetics. Transient disruption of the normal function of a gene during development can be achieved by microinjecting mRNA, DNA or short chemically stabilized anti-sense oligomers, called morpholinos (MOs), into early zebrafish embryos. The ensuing develop ment of the microinjected embryos is observed over the following hours and days to analyze the impact of the microinjected products on embryogenesis. Compared to stable reverse genetic approaches (sta ble transgenesis, targeted mutants recovered by TILLING), these transient reverse genetic approaches are vastly quicker, relatively affordable, and require little animal facility space. Common applications of these methodologies allow analysis of gain-of-function (gene overexpression or dominant active), loss-of-function (gene knock down or dominant negative), mosaic analysis, lineage-restricted studies and cell tracing experiments. The use of these transient approaches for the manipulation of gene expression has improved our understanding of many key developmental pathways including both the Wnt/β -catenin and Wnt/PCP pathways, as covered in some detail in Chapter 17 of this book. This chapter describes the most common and versatile approaches: gain of function and loss of function using DNA and mRNA injections and loss of function using MOs." @default.
- W2415393265 created "2016-06-24" @default.
- W2415393265 creator A5012092459 @default.
- W2415393265 creator A5044014434 @default.
- W2415393265 creator A5082699033 @default.
- W2415393265 creator A5082788029 @default.
- W2415393265 date "2008-01-01" @default.
- W2415393265 modified "2023-10-16" @default.
- W2415393265 title "Manipulation of Gene Expression During Zebrafish Embryonic Development Using Transient Approaches" @default.
- W2415393265 cites W1450841311 @default.
- W2415393265 cites W1495670272 @default.
- W2415393265 cites W1534052611 @default.
- W2415393265 cites W1566942630 @default.
- W2415393265 cites W1829703560 @default.
- W2415393265 cites W1901013657 @default.
- W2415393265 cites W1964743891 @default.
- W2415393265 cites W1967419775 @default.
- W2415393265 cites W1972037584 @default.
- W2415393265 cites W1973841621 @default.
- W2415393265 cites W1974660048 @default.
- W2415393265 cites W1974712344 @default.
- W2415393265 cites W1982334530 @default.
- W2415393265 cites W1983538883 @default.
- W2415393265 cites W1994858921 @default.
- W2415393265 cites W1995660054 @default.
- W2415393265 cites W1997823361 @default.
- W2415393265 cites W1998756807 @default.
- W2415393265 cites W2000865765 @default.
- W2415393265 cites W2001191599 @default.
- W2415393265 cites W2001516056 @default.
- W2415393265 cites W2001943261 @default.
- W2415393265 cites W2003699122 @default.
- W2415393265 cites W2004758097 @default.
- W2415393265 cites W2004966629 @default.
- W2415393265 cites W2005345343 @default.
- W2415393265 cites W2005462392 @default.
- W2415393265 cites W2005889545 @default.
- W2415393265 cites W2006743934 @default.
- W2415393265 cites W2009635392 @default.
- W2415393265 cites W2011002931 @default.
- W2415393265 cites W2014195226 @default.
- W2415393265 cites W2015707368 @default.
- W2415393265 cites W2017230571 @default.
- W2415393265 cites W2017567461 @default.
- W2415393265 cites W2018059970 @default.
- W2415393265 cites W2021296128 @default.
- W2415393265 cites W2021328573 @default.
- W2415393265 cites W2021945909 @default.
- W2415393265 cites W2023089317 @default.
- W2415393265 cites W2027015740 @default.
- W2415393265 cites W2027481776 @default.
- W2415393265 cites W2029222633 @default.
- W2415393265 cites W2030749989 @default.
- W2415393265 cites W2034662853 @default.
- W2415393265 cites W2034810258 @default.
- W2415393265 cites W2036211290 @default.
- W2415393265 cites W2037615799 @default.
- W2415393265 cites W2041148711 @default.
- W2415393265 cites W2041309904 @default.
- W2415393265 cites W2043127334 @default.
- W2415393265 cites W2044764655 @default.
- W2415393265 cites W2052374677 @default.
- W2415393265 cites W2064864270 @default.
- W2415393265 cites W2067109855 @default.
- W2415393265 cites W2068683659 @default.
- W2415393265 cites W2070572720 @default.
- W2415393265 cites W2071230707 @default.
- W2415393265 cites W2071285206 @default.
- W2415393265 cites W2072895744 @default.
- W2415393265 cites W2073629764 @default.
- W2415393265 cites W2074119355 @default.
- W2415393265 cites W2074911766 @default.
- W2415393265 cites W2075008830 @default.
- W2415393265 cites W2078058386 @default.
- W2415393265 cites W2078241425 @default.
- W2415393265 cites W2080646543 @default.
- W2415393265 cites W2086245382 @default.
- W2415393265 cites W2087296674 @default.
- W2415393265 cites W2091055964 @default.
- W2415393265 cites W2094530722 @default.
- W2415393265 cites W2096251733 @default.
- W2415393265 cites W2097761421 @default.
- W2415393265 cites W2101735975 @default.
- W2415393265 cites W2102072500 @default.
- W2415393265 cites W2103110972 @default.
- W2415393265 cites W2103947324 @default.
- W2415393265 cites W2103966995 @default.
- W2415393265 cites W2109686700 @default.
- W2415393265 cites W2109782739 @default.
- W2415393265 cites W2112639568 @default.
- W2415393265 cites W2113117576 @default.
- W2415393265 cites W2113187786 @default.
- W2415393265 cites W2113231837 @default.
- W2415393265 cites W2113786860 @default.
- W2415393265 cites W2118011050 @default.
- W2415393265 cites W2120841858 @default.
- W2415393265 cites W2120983879 @default.
- W2415393265 cites W2123373545 @default.