FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2415551431> ?p ?o ?g. }

• W2415551431 endingPage "100" @default.
• W2415551431 startingPage "79" @default.
• W2415551431 abstract "Lamins and associated nuclear envelope transmembrane proteins (NETs) present unique problems for biochemical studies. Lamins form insoluble intermediate filament networks, associate with chromatin, and are also connected via specific NETs to the cytoskeleton, thus further complicating their isolation and purification from mammalian cells. Adding to this complexity, NETs at the inner nuclear membrane function in three distinct environments: (a) their nucleoplasmic domain(s) can bind lamins, chromatin, and transcriptional regulators; (b) they possess one or more integral transmembrane domains; and (c) their lumenal domain(s) function in the unique reducing environment of the nuclear envelope/ER lumen. This chapter describes strategic considerations and protocols to facilitate biochemical studies of lamins and NET proteins in vitro. Studying these proteins in vitro typically involves first expressing specific polypeptide fragments in bacteria and optimizing conditions to purify each fragment. We describe parameters for choosing specific fragments and designing purification strategies and provide detailed purification protocols. Biochemical studies can provide fundamental knowledge including binding strengths and the molecular consequences of disease-causing mutations that will be essential to understand nuclear envelope–genome interactions and nuclear envelope linked disease mechanisms." @default.
• W2415551431 created "2016-06-24" @default.
• W2415551431 creator A5000535750 @default.
• W2415551431 creator A5035612494 @default.
• W2415551431 creator A5068951140 @default.
• W2415551431 creator A5078138161 @default.
• W2415551431 date "2016-01-01" @default.
• W2415551431 modified "2023-09-24" @default.
• W2415551431 title "Purification of Lamins and Soluble Fragments of NETs" @default.
• W2415551431 cites W1494579861 @default.
• W2415551431 cites W1963728629 @default.
• W2415551431 cites W1965168140 @default.
• W2415551431 cites W1969085991 @default.
• W2415551431 cites W1978660178 @default.
• W2415551431 cites W1978992677 @default.
• W2415551431 cites W1989529995 @default.
• W2415551431 cites W1993035758 @default.
• W2415551431 cites W2008900071 @default.
• W2415551431 cites W2014703919 @default.
• W2415551431 cites W2029910352 @default.
• W2415551431 cites W2030637965 @default.
• W2415551431 cites W2031920140 @default.
• W2415551431 cites W2032858454 @default.
• W2415551431 cites W2035838778 @default.
• W2415551431 cites W2050809373 @default.
• W2415551431 cites W2067761440 @default.
• W2415551431 cites W2083441358 @default.
• W2415551431 cites W2093943459 @default.
• W2415551431 cites W2095746147 @default.
• W2415551431 cites W2101083045 @default.
• W2415551431 cites W2121140096 @default.
• W2415551431 cites W2122778614 @default.
• W2415551431 cites W2128803395 @default.
• W2415551431 cites W2130912069 @default.
• W2415551431 cites W2139388482 @default.
• W2415551431 cites W2140558481 @default.
• W2415551431 cites W2141289904 @default.
• W2415551431 cites W2142780571 @default.
• W2415551431 cites W2163504716 @default.
• W2415551431 cites W2164888064 @default.
• W2415551431 cites W2165644047 @default.
• W2415551431 cites W2167960257 @default.
• W2415551431 cites W2175346537 @default.
• W2415551431 cites W2287005707 @default.
• W2415551431 cites W2305003789 @default.
• W2415551431 cites W2314179564 @default.
• W2415551431 cites W2329778565 @default.
• W2415551431 cites W4247425028 @default.
• W2415551431 cites W626315428 @default.
• W2415551431 doi "https://doi.org/10.1016/bs.mie.2015.09.006" @default.
• W2415551431 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26778554" @default.
• W2415551431 hasPublicationYear "2016" @default.
• W2415551431 type Work @default.
• W2415551431 sameAs 2415551431 @default.
• W2415551431 citedByCount "4" @default.
• W2415551431 countsByYear W24155514312019 @default.
• W2415551431 countsByYear W24155514312021 @default.
• W2415551431 crossrefType "book-chapter" @default.
• W2415551431 hasAuthorship W2415551431A5000535750 @default.
• W2415551431 hasAuthorship W2415551431A5035612494 @default.
• W2415551431 hasAuthorship W2415551431A5068951140 @default.
• W2415551431 hasAuthorship W2415551431A5078138161 @default.
• W2415551431 hasConcept C104317684 @default.
• W2415551431 hasConcept C14036430 @default.
• W2415551431 hasConcept C142669718 @default.
• W2415551431 hasConcept C1491633281 @default.
• W2415551431 hasConcept C170493617 @default.
• W2415551431 hasConcept C179093185 @default.
• W2415551431 hasConcept C24530287 @default.
• W2415551431 hasConcept C2780723820 @default.
• W2415551431 hasConcept C28859421 @default.
• W2415551431 hasConcept C29512474 @default.
• W2415551431 hasConcept C55493867 @default.
• W2415551431 hasConcept C65871279 @default.
• W2415551431 hasConcept C70721500 @default.
• W2415551431 hasConcept C83640560 @default.
• W2415551431 hasConcept C86339819 @default.
• W2415551431 hasConcept C86803240 @default.
• W2415551431 hasConcept C94912109 @default.
• W2415551431 hasConcept C95444343 @default.
• W2415551431 hasConceptScore W2415551431C104317684 @default.
• W2415551431 hasConceptScore W2415551431C14036430 @default.
• W2415551431 hasConceptScore W2415551431C142669718 @default.
• W2415551431 hasConceptScore W2415551431C1491633281 @default.
• W2415551431 hasConceptScore W2415551431C170493617 @default.
• W2415551431 hasConceptScore W2415551431C179093185 @default.
• W2415551431 hasConceptScore W2415551431C24530287 @default.
• W2415551431 hasConceptScore W2415551431C2780723820 @default.
• W2415551431 hasConceptScore W2415551431C28859421 @default.
• W2415551431 hasConceptScore W2415551431C29512474 @default.
• W2415551431 hasConceptScore W2415551431C55493867 @default.
• W2415551431 hasConceptScore W2415551431C65871279 @default.
• W2415551431 hasConceptScore W2415551431C70721500 @default.
• W2415551431 hasConceptScore W2415551431C83640560 @default.
• W2415551431 hasConceptScore W2415551431C86339819 @default.
• W2415551431 hasConceptScore W2415551431C86803240 @default.
• W2415551431 hasConceptScore W2415551431C94912109 @default.
• W2415551431 hasConceptScore W2415551431C95444343 @default.

• next