FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2415673642> ?p ?o ?g. }

• W2415673642 endingPage "1018" @default.
• W2415673642 startingPage "1005" @default.
• W2415673642 abstract "Abstract The murine monoclonal antiplatelet antibodies MA-TSPI-1 (directed against human thrombospondin) and MA-PMI-2, MA-PMI-1, and MA-LIBS-1 (directed against ligand-induced binding sites [LIBS] on human platelet glycoprotein IIb/IIIa) were conjugated with recombinant single-chain urokinase-type plasminogen activator (rscu-PA) using the cross-linking reagent N-succinimidyl 3-(2-pyridyldithio)propionate (SPDP). The conjugates (rscu-PA/MA-TSPI-1, rscu-PA/MA-PMI-2, rscu-PA/MA-PMI-1, and rscu-PA/MA-LIBS-1), purified by immunoadsorption and gel filtration, were obtained with recoveries of 34% to 45%, with an average stoichiometry of 1.6 to 1.8 IgG molecules per rscu-PA molecule, and with unaltered specific activities and affinities. Preincubation of human platelet-rich plasma with rscu-PA/MA-PMI-2, rscu-PA/MA-PMI-1, or unconjugated rscu-PA resulted in partial inhibition of ADP-induced aggregation; 25% inhibition was obtained with 63 micrograms/mL rscu-PA and with 6 micrograms u-PA/mL rscu-PA/MA-PMI-2 or 1.2 micrograms u- PA/mL rscu-PA/MA-PMI-1. In an in vitro system composed of a 125I-fibrin- labeled platelet-rich human plasma clot immersed in normal human plasma, the conjugates had threefold to greater than 15-fold less fibrinolytic potency than unconjugated rscu-PA. The thrombolytic potency of rscu-PA/MA-PMI-1 and rscu-PA/MA-LIBS-1 was compared with that of rscu-PA and that of a control conjugate rscu-PA/MA-1C8 in a pulmonary embolism model in the hamster, using clots prepared from platelet-poor or platelet-rich human plasma. Lysis was measured 30 minutes after the end of a 60-minute intravenous infusion of the thrombolytic agents. rscu-PA, rscu-PA/MA-PMI-1, rscu-PA/MA-LIBS-1, as well as rscu-PA/MA-1C8 had comparable thrombolytic potencies (percent lysis per dose administered) towards platelet-poor human plasma clots. In contrast, the thrombolytic potency of rscu-PA/MA-PMI-1 and of rscu- PA/MA-LIBS-1 towards platelet-rich clots was 2.3- to 3-fold higher than that of rscu-PA (P less than .005) and fivefold to sevenfold higher than that of the control conjugate (P less than .01)." @default.
• W2415673642 created "2016-06-24" @default.
• W2415673642 creator A5023982501 @default.
• W2415673642 creator A5036260629 @default.
• W2415673642 creator A5044752890 @default.
• W2415673642 creator A5046978879 @default.
• W2415673642 creator A5051232840 @default.
• W2415673642 creator A5054746181 @default.
• W2415673642 creator A5071619792 @default.
• W2415673642 creator A5087433287 @default.
• W2415673642 date "1991-08-15" @default.
• W2415673642 modified "2023-09-27" @default.
• W2415673642 title "Effect of chemical conjugation of recombinant single-chain urokinase- type plasminogen activator with monoclonal antiplatelet antibodies on platelet aggregation and on plasma clot lysis in vitro and in vivo" @default.
• W2415673642 cites W1495467917 @default.
• W2415673642 cites W1520666812 @default.
• W2415673642 cites W1667697323 @default.
• W2415673642 cites W1773975057 @default.
• W2415673642 cites W2000276981 @default.
• W2415673642 cites W2003364720 @default.
• W2415673642 cites W2021437817 @default.
• W2415673642 cites W2040041623 @default.
• W2415673642 cites W2045905507 @default.
• W2415673642 cites W2051512141 @default.
• W2415673642 cites W2056217168 @default.
• W2415673642 cites W2059543787 @default.
• W2415673642 cites W2075924144 @default.
• W2415673642 cites W2076134563 @default.
• W2415673642 cites W2076407505 @default.
• W2415673642 cites W2078249304 @default.
• W2415673642 cites W2080580789 @default.
• W2415673642 cites W2081576889 @default.
• W2415673642 cites W2087480868 @default.
• W2415673642 cites W2098886638 @default.
• W2415673642 cites W2101108802 @default.
• W2415673642 cites W2162806626 @default.
• W2415673642 cites W2405999289 @default.
• W2415673642 cites W2415114669 @default.
• W2415673642 cites W2419999215 @default.
• W2415673642 cites W2430593758 @default.
• W2415673642 cites W2971263105 @default.
• W2415673642 doi "https://doi.org/10.1182/blood.v78.4.1005.1005" @default.
• W2415673642 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1831057" @default.
• W2415673642 hasPublicationYear "1991" @default.
• W2415673642 type Work @default.
• W2415673642 sameAs 2415673642 @default.
• W2415673642 citedByCount "27" @default.
• W2415673642 countsByYear W24156736422012 @default.
• W2415673642 countsByYear W24156736422014 @default.
• W2415673642 countsByYear W24156736422015 @default.
• W2415673642 countsByYear W24156736422017 @default.
• W2415673642 countsByYear W24156736422020 @default.
• W2415673642 countsByYear W24156736422022 @default.
• W2415673642 crossrefType "journal-article" @default.
• W2415673642 hasAuthorship W2415673642A5023982501 @default.
• W2415673642 hasAuthorship W2415673642A5036260629 @default.
• W2415673642 hasAuthorship W2415673642A5044752890 @default.
• W2415673642 hasAuthorship W2415673642A5046978879 @default.
• W2415673642 hasAuthorship W2415673642A5051232840 @default.
• W2415673642 hasAuthorship W2415673642A5054746181 @default.
• W2415673642 hasAuthorship W2415673642A5071619792 @default.
• W2415673642 hasAuthorship W2415673642A5087433287 @default.
• W2415673642 hasBestOaLocation W24156736421 @default.
• W2415673642 hasConcept C104317684 @default.
• W2415673642 hasConcept C134018914 @default.
• W2415673642 hasConcept C153911025 @default.
• W2415673642 hasConcept C159654299 @default.
• W2415673642 hasConcept C185592680 @default.
• W2415673642 hasConcept C202751555 @default.
• W2415673642 hasConcept C203014093 @default.
• W2415673642 hasConcept C2779679481 @default.
• W2415673642 hasConcept C40767141 @default.
• W2415673642 hasConcept C542903549 @default.
• W2415673642 hasConcept C55493867 @default.
• W2415673642 hasConcept C57992300 @default.
• W2415673642 hasConcept C86803240 @default.
• W2415673642 hasConceptScore W2415673642C104317684 @default.
• W2415673642 hasConceptScore W2415673642C134018914 @default.
• W2415673642 hasConceptScore W2415673642C153911025 @default.
• W2415673642 hasConceptScore W2415673642C159654299 @default.
• W2415673642 hasConceptScore W2415673642C185592680 @default.
• W2415673642 hasConceptScore W2415673642C202751555 @default.
• W2415673642 hasConceptScore W2415673642C203014093 @default.
• W2415673642 hasConceptScore W2415673642C2779679481 @default.
• W2415673642 hasConceptScore W2415673642C40767141 @default.
• W2415673642 hasConceptScore W2415673642C542903549 @default.
• W2415673642 hasConceptScore W2415673642C55493867 @default.
• W2415673642 hasConceptScore W2415673642C57992300 @default.
• W2415673642 hasConceptScore W2415673642C86803240 @default.
• W2415673642 hasIssue "4" @default.
• W2415673642 hasLocation W24156736421 @default.
• W2415673642 hasOpenAccess W2415673642 @default.
• W2415673642 hasPrimaryLocation W24156736421 @default.
• W2415673642 hasRelatedWork W2062588824 @default.
• W2415673642 hasRelatedWork W2343297415 @default.
• W2415673642 hasRelatedWork W2360091834 @default.
• W2415673642 hasRelatedWork W2367313888 @default.
• W2415673642 hasRelatedWork W2368460920 @default.
• W2415673642 hasRelatedWork W2380360044 @default.

• next