Matches in SemOpenAlex for { <https://semopenalex.org/work/W2415690825> ?p ?o ?g. }
Showing items 1 to 82 of
82
with 100 items per page.
- W2415690825 endingPage "95" @default.
- W2415690825 startingPage "79" @default.
- W2415690825 abstract "The following DNA fragments were studied: T5A5, U5G5, C5G5, RI (CCGAATTCGG), and RV (CCGATATCGG). The ligated decamers were run on 8% polyacrylamide gel. The PAGE anomaly was measured by the R-factor. Under standard conditions R is maximum for A5G5 and U5A5 (R = 1.9-2.0); R has intermediate values for RI and T5A5 (R200 = 1.05-1.10). T5A5 exhibits unusual non-monotonous temperature dependence of the R-factor, which reaches its maximum at 35 degrees C. Based on this fact we make two conclusions: (i) PAGE anomaly is highly sensitive to the DNA winding angle tau, (ii) tau = 36 degrees for T5A5 under these conditions. For A5G5, RI and RV the temperature dependence of the R-factor is conventional: R monotonously decreases. Conceivably tau less than 36 degrees for these sequences. In the presence of 10 mM of MgCl2 the anomaly increase for all fragments, except for T5A5; e.g. for C5G5R150 R200 = 1.2. This testifies to the moderate curvature of C5G5. The results of the measurements were interpreted on the basis of the wedge AA and junction An/B models. To get a better agreement with the experiment these models should be supplemented with the bends in the YR dimers (TA, CG, CA) directed into the major groove and with the bends in AT directed into the minor groove." @default.
- W2415690825 created "2016-06-24" @default.
- W2415690825 creator A5043629505 @default.
- W2415690825 creator A5059712696 @default.
- W2415690825 creator A5083690114 @default.
- W2415690825 creator A5088471555 @default.
- W2415690825 date "1990-01-01" @default.
- W2415690825 modified "2023-09-23" @default.
- W2415690825 title "[The effect of temperature and ionic strength on the electrophoretic motility of synthetic DNA fragments]." @default.
- W2415690825 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2161494" @default.
- W2415690825 hasPublicationYear "1990" @default.
- W2415690825 type Work @default.
- W2415690825 sameAs 2415690825 @default.
- W2415690825 citedByCount "8" @default.
- W2415690825 crossrefType "journal-article" @default.
- W2415690825 hasAuthorship W2415690825A5043629505 @default.
- W2415690825 hasAuthorship W2415690825A5059712696 @default.
- W2415690825 hasAuthorship W2415690825A5083690114 @default.
- W2415690825 hasAuthorship W2415690825A5088471555 @default.
- W2415690825 hasConcept C113196181 @default.
- W2415690825 hasConcept C120665830 @default.
- W2415690825 hasConcept C121332964 @default.
- W2415690825 hasConcept C12997251 @default.
- W2415690825 hasConcept C147789679 @default.
- W2415690825 hasConcept C184651966 @default.
- W2415690825 hasConcept C185592680 @default.
- W2415690825 hasConcept C187428577 @default.
- W2415690825 hasConcept C191897082 @default.
- W2415690825 hasConcept C192562407 @default.
- W2415690825 hasConcept C195065555 @default.
- W2415690825 hasConcept C2524010 @default.
- W2415690825 hasConcept C26873012 @default.
- W2415690825 hasConcept C2779472054 @default.
- W2415690825 hasConcept C33923547 @default.
- W2415690825 hasConcept C40250595 @default.
- W2415690825 hasConcept C43617362 @default.
- W2415690825 hasConcept C47422493 @default.
- W2415690825 hasConcept C552990157 @default.
- W2415690825 hasConcept C55493867 @default.
- W2415690825 hasConcept C8010536 @default.
- W2415690825 hasConceptScore W2415690825C113196181 @default.
- W2415690825 hasConceptScore W2415690825C120665830 @default.
- W2415690825 hasConceptScore W2415690825C121332964 @default.
- W2415690825 hasConceptScore W2415690825C12997251 @default.
- W2415690825 hasConceptScore W2415690825C147789679 @default.
- W2415690825 hasConceptScore W2415690825C184651966 @default.
- W2415690825 hasConceptScore W2415690825C185592680 @default.
- W2415690825 hasConceptScore W2415690825C187428577 @default.
- W2415690825 hasConceptScore W2415690825C191897082 @default.
- W2415690825 hasConceptScore W2415690825C192562407 @default.
- W2415690825 hasConceptScore W2415690825C195065555 @default.
- W2415690825 hasConceptScore W2415690825C2524010 @default.
- W2415690825 hasConceptScore W2415690825C26873012 @default.
- W2415690825 hasConceptScore W2415690825C2779472054 @default.
- W2415690825 hasConceptScore W2415690825C33923547 @default.
- W2415690825 hasConceptScore W2415690825C40250595 @default.
- W2415690825 hasConceptScore W2415690825C43617362 @default.
- W2415690825 hasConceptScore W2415690825C47422493 @default.
- W2415690825 hasConceptScore W2415690825C552990157 @default.
- W2415690825 hasConceptScore W2415690825C55493867 @default.
- W2415690825 hasConceptScore W2415690825C8010536 @default.
- W2415690825 hasIssue "1" @default.
- W2415690825 hasLocation W24156908251 @default.
- W2415690825 hasOpenAccess W2415690825 @default.
- W2415690825 hasPrimaryLocation W24156908251 @default.
- W2415690825 hasRelatedWork W1969041273 @default.
- W2415690825 hasRelatedWork W1992237760 @default.
- W2415690825 hasRelatedWork W2017017495 @default.
- W2415690825 hasRelatedWork W2037768106 @default.
- W2415690825 hasRelatedWork W2055802510 @default.
- W2415690825 hasRelatedWork W2130062232 @default.
- W2415690825 hasRelatedWork W2186916217 @default.
- W2415690825 hasRelatedWork W2192758799 @default.
- W2415690825 hasRelatedWork W2415690825 @default.
- W2415690825 hasRelatedWork W2896898787 @default.
- W2415690825 hasVolume "24" @default.
- W2415690825 isParatext "false" @default.
- W2415690825 isRetracted "false" @default.
- W2415690825 magId "2415690825" @default.
- W2415690825 workType "article" @default.