Matches in SemOpenAlex for { <https://semopenalex.org/work/W2415697079> ?p ?o ?g. }
Showing items 1 to 77 of
77
with 100 items per page.
- W2415697079 endingPage "9" @default.
- W2415697079 startingPage "33" @default.
- W2415697079 abstract "The purpose of this study was to investigate the potential of Mesenchymal Stem Cells (MSCs) obtained from patients suffering from fractures to proliferate and differentiate towards osteogenic lineage with the use of autologous serum. In addition the effect of medium supplementation with the use of autologous serum obtained at different time points (patients' admission, first, third and seventh post-operative day) was investigated. In total eight patients suffering from lower limb long bone fractures with mean age of 39 (range 22-68 years) were included in this study. MSCs were isolated and cultivated in 10% of either Fetal Calf Serum (FCS) or autologous serum. Cellular proliferation was examined by XTT assay and Vybrant assay. The osteogenic differentiation was assessed by total calcium production and alkaline phosphatase production. Cellular proliferation and osteogenic differentiation was significantly statistically higher in patients' serum obtained on admission than in FCS. A negative effect on proliferation was noted with serum obtained on the first postoperative day. Subsequently, both proliferation and differentiation were gradually increased with autologous serum collected during the 3rd and 7th postoperatively days. Autologous serum obtained after fracture is superior in terms of proliferation and osteogenic differentiation to the currently used FCS. Surgery seems to have a negative effect on the quality of serum. These findings should be considered in cases where ex-vivo expansion of MSCs is needed. Recuperation of serum's quality takes place at a later time point within the first weeks after fracture." @default.
- W2415697079 created "2016-06-24" @default.
- W2415697079 creator A5001435316 @default.
- W2415697079 creator A5019843763 @default.
- W2415697079 creator A5022762944 @default.
- W2415697079 date "2008-10-26" @default.
- W2415697079 modified "2023-09-23" @default.
- W2415697079 title "The effect of autologous serum obtained after fracture on the proliferation and osteogenic differentiation of mesenchymal stem cells." @default.
- W2415697079 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18954549" @default.
- W2415697079 hasPublicationYear "2008" @default.
- W2415697079 type Work @default.
- W2415697079 sameAs 2415697079 @default.
- W2415697079 citedByCount "3" @default.
- W2415697079 countsByYear W24156970792018 @default.
- W2415697079 countsByYear W24156970792019 @default.
- W2415697079 countsByYear W24156970792022 @default.
- W2415697079 crossrefType "journal-article" @default.
- W2415697079 hasAuthorship W2415697079A5001435316 @default.
- W2415697079 hasAuthorship W2415697079A5019843763 @default.
- W2415697079 hasAuthorship W2415697079A5022762944 @default.
- W2415697079 hasConcept C141071460 @default.
- W2415697079 hasConcept C142724271 @default.
- W2415697079 hasConcept C160160445 @default.
- W2415697079 hasConcept C16685009 @default.
- W2415697079 hasConcept C181199279 @default.
- W2415697079 hasConcept C198826908 @default.
- W2415697079 hasConcept C203014093 @default.
- W2415697079 hasConcept C28328180 @default.
- W2415697079 hasConcept C55493867 @default.
- W2415697079 hasConcept C62112901 @default.
- W2415697079 hasConcept C71924100 @default.
- W2415697079 hasConcept C86803240 @default.
- W2415697079 hasConcept C95444343 @default.
- W2415697079 hasConceptScore W2415697079C141071460 @default.
- W2415697079 hasConceptScore W2415697079C142724271 @default.
- W2415697079 hasConceptScore W2415697079C160160445 @default.
- W2415697079 hasConceptScore W2415697079C16685009 @default.
- W2415697079 hasConceptScore W2415697079C181199279 @default.
- W2415697079 hasConceptScore W2415697079C198826908 @default.
- W2415697079 hasConceptScore W2415697079C203014093 @default.
- W2415697079 hasConceptScore W2415697079C28328180 @default.
- W2415697079 hasConceptScore W2415697079C55493867 @default.
- W2415697079 hasConceptScore W2415697079C62112901 @default.
- W2415697079 hasConceptScore W2415697079C71924100 @default.
- W2415697079 hasConceptScore W2415697079C86803240 @default.
- W2415697079 hasConceptScore W2415697079C95444343 @default.
- W2415697079 hasIssue "1" @default.
- W2415697079 hasLocation W24156970791 @default.
- W2415697079 hasOpenAccess W2415697079 @default.
- W2415697079 hasPrimaryLocation W24156970791 @default.
- W2415697079 hasRelatedWork W1978059568 @default.
- W2415697079 hasRelatedWork W2025476044 @default.
- W2415697079 hasRelatedWork W2028012976 @default.
- W2415697079 hasRelatedWork W2105734762 @default.
- W2415697079 hasRelatedWork W2118282149 @default.
- W2415697079 hasRelatedWork W2152512073 @default.
- W2415697079 hasRelatedWork W2348636174 @default.
- W2415697079 hasRelatedWork W2349557905 @default.
- W2415697079 hasRelatedWork W2351130302 @default.
- W2415697079 hasRelatedWork W2351333810 @default.
- W2415697079 hasRelatedWork W2355217822 @default.
- W2415697079 hasRelatedWork W2357106463 @default.
- W2415697079 hasRelatedWork W2361521713 @default.
- W2415697079 hasRelatedWork W2375609734 @default.
- W2415697079 hasRelatedWork W2384488912 @default.
- W2415697079 hasRelatedWork W2393693129 @default.
- W2415697079 hasRelatedWork W2502752513 @default.
- W2415697079 hasRelatedWork W2526002028 @default.
- W2415697079 hasRelatedWork W2779981387 @default.
- W2415697079 hasRelatedWork W2474037015 @default.
- W2415697079 hasVolume "54" @default.
- W2415697079 isParatext "false" @default.
- W2415697079 isRetracted "false" @default.
- W2415697079 magId "2415697079" @default.
- W2415697079 workType "article" @default.