Matches in SemOpenAlex for { <https://semopenalex.org/work/W2415702811> ?p ?o ?g. }
Showing items 1 to 78 of
78
with 100 items per page.
- W2415702811 endingPage "8" @default.
- W2415702811 startingPage "236" @default.
- W2415702811 abstract "To explore use of retroviral vector in gene therapy of hepatitis B.The recombinant vector Plxsn-HBs was constructed by inserting HBV S gene into pLXSN. The pseudovirus, which was produced from PA317 after transferring with pLXSN-HBs by electroporation, were frozen at different temperature. The activities of the pseudovirus to infect eukaryotic cells and express antigen were determined by comparing the numbers of G418-resistant clones and assaying HBsAg in the supernatant of the cells with ELISA after infection HepG2, NIH3T3 and 293 cells.It was hard to find changes in HBsAg amount at different intervals and different temperatures. G418 resistant clones, however, were variable. When frozen at -20 degrees C, the numbers of clones were half less than that of the beginning after 6 months, few clones were formed after 12 months, and no clone was found after 24 months. When frozen at -40 degrees C, the numbers of clones were 121, 332 and 89 42, 137 and 43 for HepG2, NIH3T3 and 293 cell lines at 12 and 24 months, respectively. When frozen at -70 degrees C, the numbers of clones were 159 463 and 112 for HepG2, NIH3T3 and 293 cell lines at 24 months, respectively. There was no statistical difference compared to that of zero months.The activity of the peseudovirus to infect eukaryotic cells and expressed antigen was not changed after 2 years frozen at -70 degrees C." @default.
- W2415702811 created "2016-06-24" @default.
- W2415702811 creator A5002918744 @default.
- W2415702811 creator A5020243059 @default.
- W2415702811 creator A5064811773 @default.
- W2415702811 creator A5086632320 @default.
- W2415702811 creator A5091317434 @default.
- W2415702811 date "2002-09-01" @default.
- W2415702811 modified "2023-09-23" @default.
- W2415702811 title "[Retroviral vector-mediated HBsAg expression and its stability]." @default.
- W2415702811 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12665928" @default.
- W2415702811 hasPublicationYear "2002" @default.
- W2415702811 type Work @default.
- W2415702811 sameAs 2415702811 @default.
- W2415702811 citedByCount "0" @default.
- W2415702811 crossrefType "journal-article" @default.
- W2415702811 hasAuthorship W2415702811A5002918744 @default.
- W2415702811 hasAuthorship W2415702811A5020243059 @default.
- W2415702811 hasAuthorship W2415702811A5064811773 @default.
- W2415702811 hasAuthorship W2415702811A5086632320 @default.
- W2415702811 hasAuthorship W2415702811A5091317434 @default.
- W2415702811 hasConcept C104317684 @default.
- W2415702811 hasConcept C111599444 @default.
- W2415702811 hasConcept C147483822 @default.
- W2415702811 hasConcept C153911025 @default.
- W2415702811 hasConcept C159047783 @default.
- W2415702811 hasConcept C186852380 @default.
- W2415702811 hasConcept C203014093 @default.
- W2415702811 hasConcept C2522874641 @default.
- W2415702811 hasConcept C2777410769 @default.
- W2415702811 hasConcept C2780593183 @default.
- W2415702811 hasConcept C32470452 @default.
- W2415702811 hasConcept C40767141 @default.
- W2415702811 hasConcept C54009773 @default.
- W2415702811 hasConcept C54355233 @default.
- W2415702811 hasConcept C81089528 @default.
- W2415702811 hasConcept C81885089 @default.
- W2415702811 hasConcept C86803240 @default.
- W2415702811 hasConcept C92087593 @default.
- W2415702811 hasConceptScore W2415702811C104317684 @default.
- W2415702811 hasConceptScore W2415702811C111599444 @default.
- W2415702811 hasConceptScore W2415702811C147483822 @default.
- W2415702811 hasConceptScore W2415702811C153911025 @default.
- W2415702811 hasConceptScore W2415702811C159047783 @default.
- W2415702811 hasConceptScore W2415702811C186852380 @default.
- W2415702811 hasConceptScore W2415702811C203014093 @default.
- W2415702811 hasConceptScore W2415702811C2522874641 @default.
- W2415702811 hasConceptScore W2415702811C2777410769 @default.
- W2415702811 hasConceptScore W2415702811C2780593183 @default.
- W2415702811 hasConceptScore W2415702811C32470452 @default.
- W2415702811 hasConceptScore W2415702811C40767141 @default.
- W2415702811 hasConceptScore W2415702811C54009773 @default.
- W2415702811 hasConceptScore W2415702811C54355233 @default.
- W2415702811 hasConceptScore W2415702811C81089528 @default.
- W2415702811 hasConceptScore W2415702811C81885089 @default.
- W2415702811 hasConceptScore W2415702811C86803240 @default.
- W2415702811 hasConceptScore W2415702811C92087593 @default.
- W2415702811 hasIssue "3" @default.
- W2415702811 hasLocation W24157028111 @default.
- W2415702811 hasOpenAccess W2415702811 @default.
- W2415702811 hasPrimaryLocation W24157028111 @default.
- W2415702811 hasRelatedWork W2222114301 @default.
- W2415702811 hasRelatedWork W2352258017 @default.
- W2415702811 hasRelatedWork W2358942953 @default.
- W2415702811 hasRelatedWork W2360337703 @default.
- W2415702811 hasRelatedWork W2385139158 @default.
- W2415702811 hasRelatedWork W2385863637 @default.
- W2415702811 hasRelatedWork W2389755511 @default.
- W2415702811 hasRelatedWork W2976121011 @default.
- W2415702811 hasRelatedWork W3142015144 @default.
- W2415702811 hasRelatedWork W3151405750 @default.
- W2415702811 hasVolume "16" @default.
- W2415702811 isParatext "false" @default.
- W2415702811 isRetracted "false" @default.
- W2415702811 magId "2415702811" @default.
- W2415702811 workType "article" @default.