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- W2415987148 abstract "Summary Little is known about the transport mechanism of intracellular auxin. Here, we report two vacuole‐localized proteins, Oryza sativa Os CONTINUOUS VASCULAR RING ‐ LIKE 1 (Os COLE 1) and Os COLE 1‐ INTERACTING PROTEIN (Os CLIP ), that regulate intracellular auxin transport and homoeostasis. Overexpression of Os COLE 1 markedly increased the internode length and auxin content of the stem base, whereas these parameters were decreased in RNA interference ( RNA i) plants. Os COLE 1 was localized on the tonoplast and preferentially expressed in mature tissues. We further identified its interacting protein Os CLIP , which was co‐localized on the tonoplast. Protein−protein binding assays demonstrated that the N‐terminus of Os COLE 1 directly interacted with Os CLIP in yeast cells and the rice protoplast. Furthermore, 3 H‐indole‐3‐acetic acid ( 3 H‐ IAA ) transport assays revealed that Os CLIP transported IAA into yeast cells, which was promoted by Os COLE 1. The results indicate that Os COLE 1 affects rice development by regulating intracellular auxin transport through interaction with Os CLIP , which provides a new insight into the regulatory mechanism of intracellular transport of auxin and the roles of vacuoles in plant development." @default.
- W2415987148 created "2016-06-24" @default.
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- W2415987148 date "2016-06-06" @default.
- W2415987148 modified "2023-10-05" @default.
- W2415987148 title "Interactions of <i>Oryza sativa</i> Os<scp>CONTINUOUS VASCULAR RING</scp>‐<scp>LIKE</scp> 1 (Os<scp>COLE</scp>1) and Os<scp>COLE</scp>1‐<scp>INTERACTING PROTEIN</scp> reveal a novel intracellular auxin transport mechanism" @default.
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- W2415987148 doi "https://doi.org/10.1111/nph.14021" @default.
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