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• W2415991698 abstract "Definitive endoderm (DE) gives rise to the respiratory apparatus and digestive tract. Sox17 and Cxcr4 are useful markers of the DE. Previously, we identified a novel DE marker, Decay accelerating factor 1(Daf1/CD55), by identifying DE specific genes from the expression profile of DE derived from mouse embryonic stem cells (ESCs) by microarray analysis, and in situ hybridization of early embryos. Daf1 is expressed in a subpopulation of E-cadherin + Cxcr4+ DE cells. The characteristics of the Daf1-expressing cells during DE differentiation has not been examined.In this report, we utilized the ESC differentiation system to examine the characteristics of Daf1-expressing DE cells. We found that Daf1 expression could discriminate late DE from early DE. Early DE cells are Daf1-negative (DE-) and late DE cells are Daf1-positive (DE+). We also found that Daf1+ late DE cells show low proliferative and low cell matrix adhesive characteristics. Furthermore, the purified SOX17(low) early DE cells gave rise to Daf1+ Sox17(high) late DE cells.Daf1-expressing late definitive endoderm proliferates slowly and show low adhesive capacity." @default.
• W2415991698 created "2016-06-24" @default.
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• W2415991698 date "2016-05-31" @default.
• W2415991698 modified "2023-10-17" @default.
• W2415991698 title "Late stage definitive endodermal differentiation can be defined by Daf1 expression" @default.
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• W2415991698 doi "https://doi.org/10.1186/s12861-016-0120-2" @default.
• W2415991698 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4888667" @default.
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• W2415991698 hasPublicationYear "2016" @default.
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