Matches in SemOpenAlex for { <https://semopenalex.org/work/W2416013857> ?p ?o ?g. }
Showing items 1 to 77 of
77
with 100 items per page.
- W2416013857 endingPage "13" @default.
- W2416013857 startingPage "805" @default.
- W2416013857 abstract "The cross-bridge cycle and related kinetic parameters in insect muscles is determined in a skinned fiber system. Small preparations consisting of 2 fibers each are isolated from indirect flight muscles from a giant water bug, Lethocerus Colossicus, and used for experiments. These preparations have stretch activation with the rate constant parameters becoming stable at 5% above the slack length and longer. The preparations are activated in the presence of (mM:) 6 CaEGTA (pCa 4.5), 1 Mg++, 10 NaN3, 10 MOPS (pH 7.00), 15 creatine phosphate, 350 unit/ml kinase, 0.05-5mM MgATP, 0-16mM phosphate, and the ionic strength adjusted to 140mM with K proprionate. The kinetic parameters are assessed by small amplitude (0.25%) length oscillations at varying frequencies (0.25-350Hz), and by following amplitude and phase shift in tension. From these, the complex modulus is determined by the ratio of the stress to the strain in the frequency domain. Exponential processes (B) and (C) are identified in the complex modulus data; process (A) is very small or absent. Process (B) is known as oscillatory work, and is equivalent to delayed tension in step analysis. Process (C) is equivalent to phase 2 of the same. When the MgATP concentration is increased, the rate constants of both processes (B) and (C) increase and saturate. With increasing concentrations of phosphate, the rate constant of processes (B) also increases. The MgATP and phosphate dependence of the rate constants are analyzed in terms of a cross-bridge scheme with 5 states, and the following kinetic parameters are deduced: K1 = 0.7mM-1, k2 = 900s-1, k-2 = 180s-1, K2 = 5, and k3 = 70s-1.(ABSTRACT TRUNCATED AT 250 WORDS)" @default.
- W2416013857 created "2016-06-24" @default.
- W2416013857 creator A5064087943 @default.
- W2416013857 creator A5077837875 @default.
- W2416013857 date "1990-01-01" @default.
- W2416013857 modified "2023-10-18" @default.
- W2416013857 title "Role of MgATP and inorganic phosphate ions in cross-bridge kinetics in insect (Lethocerus colossicus) flight muscle." @default.
- W2416013857 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2138792" @default.
- W2416013857 hasPublicationYear "1990" @default.
- W2416013857 type Work @default.
- W2416013857 sameAs 2416013857 @default.
- W2416013857 citedByCount "3" @default.
- W2416013857 crossrefType "journal-article" @default.
- W2416013857 hasAuthorship W2416013857A5064087943 @default.
- W2416013857 hasAuthorship W2416013857A5077837875 @default.
- W2416013857 hasConcept C113196181 @default.
- W2416013857 hasConcept C121332964 @default.
- W2416013857 hasConcept C12554922 @default.
- W2416013857 hasConcept C145148216 @default.
- W2416013857 hasConcept C147789679 @default.
- W2416013857 hasConcept C148898269 @default.
- W2416013857 hasConcept C159985019 @default.
- W2416013857 hasConcept C178790620 @default.
- W2416013857 hasConcept C180016635 @default.
- W2416013857 hasConcept C184651966 @default.
- W2416013857 hasConcept C185592680 @default.
- W2416013857 hasConcept C186068551 @default.
- W2416013857 hasConcept C187428577 @default.
- W2416013857 hasConcept C192562407 @default.
- W2416013857 hasConcept C2182769 @default.
- W2416013857 hasConcept C2777132085 @default.
- W2416013857 hasConcept C43617362 @default.
- W2416013857 hasConcept C55493867 @default.
- W2416013857 hasConcept C62520636 @default.
- W2416013857 hasConcept C86803240 @default.
- W2416013857 hasConcept C93391505 @default.
- W2416013857 hasConceptScore W2416013857C113196181 @default.
- W2416013857 hasConceptScore W2416013857C121332964 @default.
- W2416013857 hasConceptScore W2416013857C12554922 @default.
- W2416013857 hasConceptScore W2416013857C145148216 @default.
- W2416013857 hasConceptScore W2416013857C147789679 @default.
- W2416013857 hasConceptScore W2416013857C148898269 @default.
- W2416013857 hasConceptScore W2416013857C159985019 @default.
- W2416013857 hasConceptScore W2416013857C178790620 @default.
- W2416013857 hasConceptScore W2416013857C180016635 @default.
- W2416013857 hasConceptScore W2416013857C184651966 @default.
- W2416013857 hasConceptScore W2416013857C185592680 @default.
- W2416013857 hasConceptScore W2416013857C186068551 @default.
- W2416013857 hasConceptScore W2416013857C187428577 @default.
- W2416013857 hasConceptScore W2416013857C192562407 @default.
- W2416013857 hasConceptScore W2416013857C2182769 @default.
- W2416013857 hasConceptScore W2416013857C2777132085 @default.
- W2416013857 hasConceptScore W2416013857C43617362 @default.
- W2416013857 hasConceptScore W2416013857C55493867 @default.
- W2416013857 hasConceptScore W2416013857C62520636 @default.
- W2416013857 hasConceptScore W2416013857C86803240 @default.
- W2416013857 hasConceptScore W2416013857C93391505 @default.
- W2416013857 hasLocation W24160138571 @default.
- W2416013857 hasOpenAccess W2416013857 @default.
- W2416013857 hasPrimaryLocation W24160138571 @default.
- W2416013857 hasRelatedWork W1978275179 @default.
- W2416013857 hasRelatedWork W1992276599 @default.
- W2416013857 hasRelatedWork W2055458776 @default.
- W2416013857 hasRelatedWork W2095182796 @default.
- W2416013857 hasRelatedWork W2169750519 @default.
- W2416013857 hasRelatedWork W2334932612 @default.
- W2416013857 hasRelatedWork W2418243278 @default.
- W2416013857 hasRelatedWork W2624890242 @default.
- W2416013857 hasRelatedWork W2950669456 @default.
- W2416013857 hasRelatedWork W115528866 @default.
- W2416013857 hasVolume "327" @default.
- W2416013857 isParatext "false" @default.
- W2416013857 isRetracted "false" @default.
- W2416013857 magId "2416013857" @default.
- W2416013857 workType "article" @default.