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- W2416016288 abstract "Enzymatic distribution in the renal homotransplants was experimentally investigated with histochemical stain technique in the course after the interventions. Changes in the enzymatic distribution were compared with those resulted from autotransplantation and temporary renal ischemia. The enzymes investigated were succinic dehydrogenase, lactic dehydrogenase, DPN diaphorase, alkaline phosphatase and acid phosphatase. 97 mongrel dogs weighing 9 to 24 Kg were divided into 4 groups, namely Groups of homotransplantation with and without immunosuppressive treatment, Group of autotransplantation and Group of temporary renal ischemia.The results are follows:1) Changes of the enzymatic distribution in autographsplants were transient and did not differ from those after temporary renal ischemia. Decreased enzymatic activity was restored to normal within 7 days.2) Until the 6th postoperative day, the changes in homotransplants without immunosuppression were alike to those of the autotransplants, the decreased enzymatic activity restoring gradually, but thereafter, after showing a slight reduction, a sudden dramatic reduction or deterioration in all enzymatic activity was observed, as soon as urine flow stopped.3) In the group of homotransplantation with immunosuppression, the changes in enzymatic patterns were postponed, showing a similar tendency to the group of homotransplantation without immunosuppression. There were no specific changes combined with Imuran administration.4) The enzymes of dehydrogenation activity showed a more marked change than the phosphatases. Above all, succinic dehydrogenase pattern in the proximal convoluted tubuli showed the most marked change.5) It was interesting that the changes in the enzymatic patterns did not run parallel to the degree of perivascular infiltration of pyroninophilic cells, which began to appear about three days after the intervention and spread thoroughly in the cortex in the stage of urine flow stoppage. The abrupt change of enzymatic activity combined with rejection phenomenon suggested that the perivascular cellular infiltration was not the sole causative factor in development of rejection phenomenon, and that the “humoral mechanism” should be also considered.6) Possibility in clinical application of histochemical technique in early detection of rejection phenomenon was discussed." @default.
- W2416016288 created "2016-06-24" @default.
- W2416016288 creator A5076829080 @default.
- W2416016288 date "1967-01-01" @default.
- W2416016288 modified "2023-09-26" @default.
- W2416016288 title "A HISTOCHEMICAL STUDY ON RENAL HOMOTRANSPLANTS" @default.
- W2416016288 doi "https://doi.org/10.5980/jpnjurol1928.58.6_608" @default.
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