Matches in SemOpenAlex for { <https://semopenalex.org/work/W2416168519> ?p ?o ?g. }
- W2416168519 endingPage "2773" @default.
- W2416168519 startingPage "2763" @default.
- W2416168519 abstract "Bone marrow-derived mesenchymal stem cells (BMSCs) can be obtained by minimally invasive means and would be a favourable source for cell-based cartilage regeneration. However, controlling the differentiation of the BMSCs towards the desired chondrogenic pathway has been a challenge hampering their application. The major aim of the present study was to determine if conditioned medium collected from cultured auricular chondrocytes could promote chondrogenic differentiation of BMSCs. Auricular chondrocytes were isolated and grown in BMSC standard culture medium (SM) that was collected and used as chondrocyte-conditioned medium (CCM). The BMSCs were expanded in either CCM or SM for three passages. Cells were seeded onto fibrous collagen scaffolds and precultured for 2 weeks with or without transforming growth factor-beta 3 (TGF-β3). After preculture, constructs were implanted subcutaneously in nude mice for 6 and 12 weeks and evaluated with real-time polymerase chain reaction, histology, immunohistochemistry and biochemistry. Real-time polymerase chain reaction results showed upregulation of COL2A1 in the constructs cultured in CCM compared with those in SM. After 12 weeks in vivo, abundant neocartilage formation was observed in the implants that had been cultured in CCM, with or without TGF-β3. In contrast, very little cartilage matrix formation was observed within the SM groups, regardless of the presence of TGF-β3. Osteogenesis was only observed in the SM group with TGF-β3. In conclusion, CCM even had a stronger influence on chondrogenesis than the supplementation of the standard culture medium with TGF-β3, without signs of endochondral ossification. Efficient chondrogenic differentiation of BMSCs could provide a promising alternative cell population for auricular regeneration. Copyright © 2016 John Wiley & Sons, Ltd." @default.
- W2416168519 created "2016-06-24" @default.
- W2416168519 creator A5030086439 @default.
- W2416168519 creator A5031180159 @default.
- W2416168519 creator A5031502367 @default.
- W2416168519 creator A5036096394 @default.
- W2416168519 creator A5042597057 @default.
- W2416168519 creator A5048238441 @default.
- W2416168519 creator A5053597612 @default.
- W2416168519 creator A5061661160 @default.
- W2416168519 creator A5066520058 @default.
- W2416168519 creator A5068730158 @default.
- W2416168519 creator A5075710060 @default.
- W2416168519 date "2016-06-03" @default.
- W2416168519 modified "2023-09-30" @default.
- W2416168519 title "Chondrogenesis by bone marrow‐derived mesenchymal stem cells grown in chondrocyte‐conditioned medium for auricular reconstruction" @default.
- W2416168519 cites W1495519011 @default.
- W2416168519 cites W1598896115 @default.
- W2416168519 cites W1968592374 @default.
- W2416168519 cites W1970738731 @default.
- W2416168519 cites W1975082513 @default.
- W2416168519 cites W1986845239 @default.
- W2416168519 cites W1988635537 @default.
- W2416168519 cites W1989027893 @default.
- W2416168519 cites W1989739945 @default.
- W2416168519 cites W1992696273 @default.
- W2416168519 cites W1999007447 @default.
- W2416168519 cites W2004290723 @default.
- W2416168519 cites W2015518562 @default.
- W2416168519 cites W2021331319 @default.
- W2416168519 cites W2025587354 @default.
- W2416168519 cites W2027736364 @default.
- W2416168519 cites W2031570779 @default.
- W2416168519 cites W2032328269 @default.
- W2416168519 cites W2037807367 @default.
- W2416168519 cites W2041242822 @default.
- W2416168519 cites W2042382626 @default.
- W2416168519 cites W2049401674 @default.
- W2416168519 cites W2054711890 @default.
- W2416168519 cites W2057465261 @default.
- W2416168519 cites W2058316419 @default.
- W2416168519 cites W2067042965 @default.
- W2416168519 cites W2068667239 @default.
- W2416168519 cites W2082308519 @default.
- W2416168519 cites W2083652435 @default.
- W2416168519 cites W2087735843 @default.
- W2416168519 cites W2092904432 @default.
- W2416168519 cites W2099048257 @default.
- W2416168519 cites W2101709664 @default.
- W2416168519 cites W2104799329 @default.
- W2416168519 cites W2107277218 @default.
- W2416168519 cites W2107428938 @default.
- W2416168519 cites W2114241729 @default.
- W2416168519 cites W2122764374 @default.
- W2416168519 cites W2126423341 @default.
- W2416168519 cites W2144880085 @default.
- W2416168519 cites W2145306093 @default.
- W2416168519 cites W2146560938 @default.
- W2416168519 cites W2166765590 @default.
- W2416168519 cites W2166980716 @default.
- W2416168519 cites W2169596632 @default.
- W2416168519 cites W2171410660 @default.
- W2416168519 cites W2208275894 @default.
- W2416168519 cites W2312279405 @default.
- W2416168519 doi "https://doi.org/10.1002/term.2171" @default.
- W2416168519 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27256796" @default.
- W2416168519 hasPublicationYear "2016" @default.
- W2416168519 type Work @default.
- W2416168519 sameAs 2416168519 @default.
- W2416168519 citedByCount "26" @default.
- W2416168519 countsByYear W24161685192017 @default.
- W2416168519 countsByYear W24161685192018 @default.
- W2416168519 countsByYear W24161685192019 @default.
- W2416168519 countsByYear W24161685192020 @default.
- W2416168519 countsByYear W24161685192021 @default.
- W2416168519 countsByYear W24161685192022 @default.
- W2416168519 countsByYear W24161685192023 @default.
- W2416168519 crossrefType "journal-article" @default.
- W2416168519 hasAuthorship W2416168519A5030086439 @default.
- W2416168519 hasAuthorship W2416168519A5031180159 @default.
- W2416168519 hasAuthorship W2416168519A5031502367 @default.
- W2416168519 hasAuthorship W2416168519A5036096394 @default.
- W2416168519 hasAuthorship W2416168519A5042597057 @default.
- W2416168519 hasAuthorship W2416168519A5048238441 @default.
- W2416168519 hasAuthorship W2416168519A5053597612 @default.
- W2416168519 hasAuthorship W2416168519A5061661160 @default.
- W2416168519 hasAuthorship W2416168519A5066520058 @default.
- W2416168519 hasAuthorship W2416168519A5068730158 @default.
- W2416168519 hasAuthorship W2416168519A5075710060 @default.
- W2416168519 hasBestOaLocation W24161685191 @default.
- W2416168519 hasConcept C105702510 @default.
- W2416168519 hasConcept C16685009 @default.
- W2416168519 hasConcept C17938293 @default.
- W2416168519 hasConcept C185592680 @default.
- W2416168519 hasConcept C198826908 @default.
- W2416168519 hasConcept C203014093 @default.
- W2416168519 hasConcept C2780007613 @default.
- W2416168519 hasConcept C2780550940 @default.