Matches in SemOpenAlex for { <https://semopenalex.org/work/W2416236444> ?p ?o ?g. }
Showing items 1 to 84 of
84
with 100 items per page.
- W2416236444 endingPage "102" @default.
- W2416236444 startingPage "98" @default.
- W2416236444 abstract "To investigate the role of miR-21 in airway immunologic dysfunction induced by cold air irritation.Immortalized human airway epithelial cell lines BEAS-2B and 16HBE cells were cultured in air-liquid phases. The differential expressions of endogenous miR-21, miR-164, and miR-155 in the cells induced by cold air exposure for different time were detected by real-time PCR. The reporter plasmid containing wild-type or mutated 3'UTR of TLR-4 were constructed and co-transfected into BEAS-2B cells or 16HBE cells together with miR-21 mimic, miR-21 mimic control, miR-21 inhibitor, or miR-21 inhibitor control. Following the transfection, dual luciferase reporter assay was performed to verify the action of miR-21 on TLR-4. miR-21 mimic, miR-21 mimic control, miR-21 inhibitor, and miR-21 inhibitor control were transfected via lipofectamine 2000 in BEAS-2B or 16HBE cells that were subsequently exposed to a temperature at 37 degrees celsius; or cold irritation (30 degrees celsius;), and the protein levels of TLR-4/MyD88 were detected by Western blotting.Cold irritation caused a time- dependent up-regulation of miR-21 in both BEAS-2B and 16HBE cells (P<0.05) without obviously affecting the expressions of miR-164 and miR-155. Dual luciferase reporter assay demonstrated a direct combination of miR-21 and its target protein TLR-4. The synthesis levels of TLR-4/MyD88 protein were decreased in miR-21 mimic group even at a routine culture temperature (P<0.05), as also seen in cells with cold irritation (P<0.05). Treatment with the miR-21 inhibitor partially attenuated cold irritation-induced down-regulation of TLR-4/MyD88 protein (P<0.05).Cold air irritation-induced airway immunologic dysfunction is probably associated with TLR-4/MyD88 down-regulation by an increased endogenic miR-21." @default.
- W2416236444 created "2016-06-24" @default.
- W2416236444 creator A5024364406 @default.
- W2416236444 creator A5039565419 @default.
- W2416236444 creator A5063112175 @default.
- W2416236444 creator A5071445293 @default.
- W2416236444 creator A5081386493 @default.
- W2416236444 date "2016-01-01" @default.
- W2416236444 modified "2023-09-24" @default.
- W2416236444 title "[Modulation of TLR-4/MyD88 signaling cascade by miR-21 is involved in airway immunologic dysfunction induced by cold air exposure]." @default.
- W2416236444 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26806747" @default.
- W2416236444 hasPublicationYear "2016" @default.
- W2416236444 type Work @default.
- W2416236444 sameAs 2416236444 @default.
- W2416236444 citedByCount "0" @default.
- W2416236444 crossrefType "journal-article" @default.
- W2416236444 hasAuthorship W2416236444A5024364406 @default.
- W2416236444 hasAuthorship W2416236444A5039565419 @default.
- W2416236444 hasAuthorship W2416236444A5063112175 @default.
- W2416236444 hasAuthorship W2416236444A5071445293 @default.
- W2416236444 hasAuthorship W2416236444A5081386493 @default.
- W2416236444 hasConcept C104317684 @default.
- W2416236444 hasConcept C121332964 @default.
- W2416236444 hasConcept C127561419 @default.
- W2416236444 hasConcept C1491633281 @default.
- W2416236444 hasConcept C150194340 @default.
- W2416236444 hasConcept C153911025 @default.
- W2416236444 hasConcept C161733203 @default.
- W2416236444 hasConcept C16613235 @default.
- W2416236444 hasConcept C185592680 @default.
- W2416236444 hasConcept C40767141 @default.
- W2416236444 hasConcept C49923114 @default.
- W2416236444 hasConcept C53227056 @default.
- W2416236444 hasConcept C54009773 @default.
- W2416236444 hasConcept C54355233 @default.
- W2416236444 hasConcept C55493867 @default.
- W2416236444 hasConcept C6425155 @default.
- W2416236444 hasConcept C81885089 @default.
- W2416236444 hasConcept C85265743 @default.
- W2416236444 hasConcept C86803240 @default.
- W2416236444 hasConcept C92087593 @default.
- W2416236444 hasConcept C97355855 @default.
- W2416236444 hasConceptScore W2416236444C104317684 @default.
- W2416236444 hasConceptScore W2416236444C121332964 @default.
- W2416236444 hasConceptScore W2416236444C127561419 @default.
- W2416236444 hasConceptScore W2416236444C1491633281 @default.
- W2416236444 hasConceptScore W2416236444C150194340 @default.
- W2416236444 hasConceptScore W2416236444C153911025 @default.
- W2416236444 hasConceptScore W2416236444C161733203 @default.
- W2416236444 hasConceptScore W2416236444C16613235 @default.
- W2416236444 hasConceptScore W2416236444C185592680 @default.
- W2416236444 hasConceptScore W2416236444C40767141 @default.
- W2416236444 hasConceptScore W2416236444C49923114 @default.
- W2416236444 hasConceptScore W2416236444C53227056 @default.
- W2416236444 hasConceptScore W2416236444C54009773 @default.
- W2416236444 hasConceptScore W2416236444C54355233 @default.
- W2416236444 hasConceptScore W2416236444C55493867 @default.
- W2416236444 hasConceptScore W2416236444C6425155 @default.
- W2416236444 hasConceptScore W2416236444C81885089 @default.
- W2416236444 hasConceptScore W2416236444C85265743 @default.
- W2416236444 hasConceptScore W2416236444C86803240 @default.
- W2416236444 hasConceptScore W2416236444C92087593 @default.
- W2416236444 hasConceptScore W2416236444C97355855 @default.
- W2416236444 hasIssue "1" @default.
- W2416236444 hasLocation W24162364441 @default.
- W2416236444 hasOpenAccess W2416236444 @default.
- W2416236444 hasPrimaryLocation W24162364441 @default.
- W2416236444 hasRelatedWork W2350572624 @default.
- W2416236444 hasRelatedWork W2358953894 @default.
- W2416236444 hasRelatedWork W2364319975 @default.
- W2416236444 hasRelatedWork W2379416539 @default.
- W2416236444 hasRelatedWork W2394952971 @default.
- W2416236444 hasRelatedWork W2410849199 @default.
- W2416236444 hasRelatedWork W2416236444 @default.
- W2416236444 hasRelatedWork W3031788429 @default.
- W2416236444 hasRelatedWork W3032845898 @default.
- W2416236444 hasRelatedWork W3032689747 @default.
- W2416236444 hasVolume "36" @default.
- W2416236444 isParatext "false" @default.
- W2416236444 isRetracted "false" @default.
- W2416236444 magId "2416236444" @default.
- W2416236444 workType "article" @default.