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- W2416400485 abstract "We describe a procedure for quantitative detection of nucleic acids by coupling PCR with improved chemiluminescent detection techniques. After performing PCR in the exponential phase in the presence of a trace amount of digoxigenin-11-dUTP, the amplified products are transferred to a positively charged nylon membrane. The membrane is cleaned with a modified method involving sodium dodecyl sulfate and ethanol washing steps to ensure low backgrounds in chemiluminescent detection. The membrane is exposed to two stacked x-ray films to increase the dynamic response in a film exposure. This sensitive and quantitative procedure is useful for molecular biology studies." @default.
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- W2416400485 date "1994-10-01" @default.
- W2416400485 modified "2023-09-27" @default.
- W2416400485 title "Optimized chemiluminescent detection of DNA amplified in the exponential phase of PCR." @default.
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