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• W2416459311 abstract "Most methods currently available for the analysis of chromatin in vivo rely on a priori knowledge of putative chromatin components or their posttranslational modification state. The isolation of defined native chromosomal regions provides an attractive alternative to obtain a largely unbiased molecular description of chromatin. Here, we describe a strategy combining site-specific recombination at the chromosome with an efficient tandem affinity purification protocol to isolate a single-copy gene locus from the yeast Saccharomyces cerevisiae. The method allows robust enrichment of a targeted chromatin domain, making it amenable to compositional, structural, and biochemical analyses. This technique appears to be suitable to obtain a detailed description of chromatin composition and specific posttranslational histone modification state at virtually any genomic locus in yeast." @default.
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• W2416459311 date "2013-10-07" @default.
• W2416459311 modified "2023-09-24" @default.
• W2416459311 title "Purification of Specific Chromatin Domains from Single-Copy Gene Loci in Saccharomyces cerevisiae" @default.
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• W2416459311 doi "https://doi.org/10.1007/978-1-62703-706-8_26" @default.
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