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- W2416509898 abstract "To compare the differences of chromosome aberration among 3 cloning cells (MC2, MC3 and MC4) of SO-Rb50 cell line.G-banding and karyotype analysis were performed on the 11th passage cells of MC2, MC3 and MC4 cell strains.Both numerical abnormal and structural aberrations of chromosomes could be observed in these 3 cloned cell strains. Diploid cells had the preponderance over the aneuploid cells. However, some cells showed pseudiploid karyotypes. Several kinds of structural chromosome aberrations were observed. The chromosome aberrations in the same passage of different cell strains were different. Aberration of chromosome 13 was rare and the aberration feature were different in 3 cloned cell strains. Five marker chromosomes were identified. M1, t(1;1) qterp35::q24-ter could be found in all cell strains. Other markers were seen in different cell strains respectively. In our experiment, two marker chromosome in chromosome 2, M4 and M5, were found in SO-Rb50 cell line for the first time.Chromosome aberrations were different with different cloned cell strains. Combined with formerly study in SO-Rb50 cell line, we found the chromosome aberration had dynamic changes during the long-term culture in vitro. We suggest that aberration of chromosome 13 is not the only cause of Rb; aberration of chromosome 1, a common event in some neoplasias as well as in SO-Rb50, plays a meaningful role in the immortalization of this cell line; other aberrations observed in our experiment could be considered as evidences of genomic instability of the cell strains and presumably enhance the growth potential of the cells in which they develop." @default.
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- W2416509898 date "1998-12-01" @default.
- W2416509898 modified "2023-09-23" @default.
- W2416509898 title "[An investigation on chromosome aberration of SO-Rb50 cloned cell strains]." @default.
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