Matches in SemOpenAlex for { <https://semopenalex.org/work/W2416529116> ?p ?o ?g. }
Showing items 1 to 78 of
78
with 100 items per page.
- W2416529116 endingPage "5" @default.
- W2416529116 startingPage "4011" @default.
- W2416529116 abstract "During recent decades, studies of various cancer-related genes has led to a growing understanding of molecular mechanisms of gastrointestinal cancer resulting in a genetic progression model. Nevertheless, with a few exceptions, our knowledge of participating genes has not been exploited for gene therapeutic approaches. Therefore, we monitored the promoter activity of genes shown to be significantly expressed in gastrointestinal tumors to select optimally active promoters for recombinant DNA constructs. Such molecules will contain a suicide gene under a suitable cell type-specific regulatory element.Using promoter-reporter gene (luciferase) constructs we compared the activities of KRT19, TFF1, SEL1L, MUC4, MUC1, CEL and hTERT by transfecting them into the gastrointestinal cell lines MKN45 and DAN-G for transient expression. Furthermore we tested the endogenous expression of these genes by RT-PCR.From a selection of 9 promoter constructs SEL1L, KRT19 and MUC1 displayed the highest activity levels while others were moderately expressed.These three appear the most capable ones to drive suicide genes, like thymidine kinase or cytosine deaminase, a study presently initiated." @default.
- W2416529116 created "2016-06-24" @default.
- W2416529116 creator A5001054532 @default.
- W2416529116 creator A5003956431 @default.
- W2416529116 creator A5031521718 @default.
- W2416529116 creator A5040743801 @default.
- W2416529116 creator A5045729000 @default.
- W2416529116 creator A5060101437 @default.
- W2416529116 creator A5079519045 @default.
- W2416529116 date "2003-12-12" @default.
- W2416529116 modified "2023-09-23" @default.
- W2416529116 title "Assessing optimal promoter activity for constructs in gastrointestinal gene therapy." @default.
- W2416529116 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/14666711" @default.
- W2416529116 hasPublicationYear "2003" @default.
- W2416529116 type Work @default.
- W2416529116 sameAs 2416529116 @default.
- W2416529116 citedByCount "2" @default.
- W2416529116 crossrefType "journal-article" @default.
- W2416529116 hasAuthorship W2416529116A5001054532 @default.
- W2416529116 hasAuthorship W2416529116A5003956431 @default.
- W2416529116 hasAuthorship W2416529116A5031521718 @default.
- W2416529116 hasAuthorship W2416529116A5040743801 @default.
- W2416529116 hasAuthorship W2416529116A5045729000 @default.
- W2416529116 hasAuthorship W2416529116A5060101437 @default.
- W2416529116 hasAuthorship W2416529116A5079519045 @default.
- W2416529116 hasConcept C101762097 @default.
- W2416529116 hasConcept C102230213 @default.
- W2416529116 hasConcept C104317684 @default.
- W2416529116 hasConcept C111335760 @default.
- W2416529116 hasConcept C111599444 @default.
- W2416529116 hasConcept C150194340 @default.
- W2416529116 hasConcept C161733203 @default.
- W2416529116 hasConcept C2522874641 @default.
- W2416529116 hasConcept C2776147627 @default.
- W2416529116 hasConcept C2777748644 @default.
- W2416529116 hasConcept C2781196997 @default.
- W2416529116 hasConcept C34981463 @default.
- W2416529116 hasConcept C502942594 @default.
- W2416529116 hasConcept C54009773 @default.
- W2416529116 hasConcept C54355233 @default.
- W2416529116 hasConcept C86803240 @default.
- W2416529116 hasConceptScore W2416529116C101762097 @default.
- W2416529116 hasConceptScore W2416529116C102230213 @default.
- W2416529116 hasConceptScore W2416529116C104317684 @default.
- W2416529116 hasConceptScore W2416529116C111335760 @default.
- W2416529116 hasConceptScore W2416529116C111599444 @default.
- W2416529116 hasConceptScore W2416529116C150194340 @default.
- W2416529116 hasConceptScore W2416529116C161733203 @default.
- W2416529116 hasConceptScore W2416529116C2522874641 @default.
- W2416529116 hasConceptScore W2416529116C2776147627 @default.
- W2416529116 hasConceptScore W2416529116C2777748644 @default.
- W2416529116 hasConceptScore W2416529116C2781196997 @default.
- W2416529116 hasConceptScore W2416529116C34981463 @default.
- W2416529116 hasConceptScore W2416529116C502942594 @default.
- W2416529116 hasConceptScore W2416529116C54009773 @default.
- W2416529116 hasConceptScore W2416529116C54355233 @default.
- W2416529116 hasConceptScore W2416529116C86803240 @default.
- W2416529116 hasIssue "5A" @default.
- W2416529116 hasLocation W24165291161 @default.
- W2416529116 hasOpenAccess W2416529116 @default.
- W2416529116 hasPrimaryLocation W24165291161 @default.
- W2416529116 hasRelatedWork W1992135196 @default.
- W2416529116 hasRelatedWork W1999807457 @default.
- W2416529116 hasRelatedWork W2009370249 @default.
- W2416529116 hasRelatedWork W2041266730 @default.
- W2416529116 hasRelatedWork W2074949958 @default.
- W2416529116 hasRelatedWork W2094314627 @default.
- W2416529116 hasRelatedWork W2187011112 @default.
- W2416529116 hasRelatedWork W2364959773 @default.
- W2416529116 hasRelatedWork W2416529116 @default.
- W2416529116 hasRelatedWork W2550225615 @default.
- W2416529116 hasVolume "23" @default.
- W2416529116 isParatext "false" @default.
- W2416529116 isRetracted "false" @default.
- W2416529116 magId "2416529116" @default.
- W2416529116 workType "article" @default.