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- W2416684311 abstract "We used cultured adult mouse retinae as a model system to follow and quantify the retraction of dendrites using diolistic labelling of retinal ganglion cells (RGCs) following explantation. Cell death was monitored in parallel by nuclear staining as 'labelling' with RGC and apoptotic markers was inconsistent and exceedingly difficult to quantify reliably. Nuclear staining allowed us to delineate a lengthy time window during which dendrite retraction can be monitored in the absence of RGC death. The addition of brain-derived neurotrophic factor (BDNF) produced a marked reduction in dendritic degeneration, even when application was delayed for 3 days after retinal explantation. These results suggest that the delayed addition of trophic factors may be functionally beneficial before the loss of cell bodies in the course of conditions such as glaucoma." @default.
- W2416684311 created "2016-06-24" @default.
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- W2416684311 date "2016-07-19" @default.
- W2416684311 modified "2023-10-14" @default.
- W2416684311 title "Brain-derived neurotrophic factor prevents dendritic retraction of adult mouse retinal ganglion cells" @default.
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- W2416684311 doi "https://doi.org/10.1111/ejn.13295" @default.
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