Matches in SemOpenAlex for { <https://semopenalex.org/work/W2416727787> ?p ?o ?g. }
Showing items 1 to 56 of
56
with 100 items per page.
- W2416727787 endingPage "7" @default.
- W2416727787 startingPage "51" @default.
- W2416727787 abstract "We have reported that sera from a majority of patients with non-insulin-dependent diabetes mellitus (NIDDM) inhibit insulin-stimulated lipogenesis (3-3H-glucose conversion to 3H-lipid) in rat adipocytes to a greater extent than control sera or sera from patients with insulin-dependent diabetes mellitus (IDDM). This effect was apparently due to a circulating, low molecular weight (Mr) substance soluble in acid/ethanol and resistant to proteases. We now describe the further characterization of this inhibitor isolated uniquely from NIDDM sera. Size exclusion chromatography of acid/ethanol extracts of sera on a Bio-Rad P2 column revealed the presence of a Mr 300-400 inhibitor of insulin-stimulated lipogenesis in 32 (70%) of 46 NIDDM sera but not in 9 IDDM or 12 control sera. NIDDM sera that contained the low Mr inhibitor had significantly elevated haemoglobin A1c levels compared with those without the inhibitor (14.1 +/- 2.6% vs 11.5 +/- 2.7%, p less than 0.001). In rat adipocytes, the low Mr inhibitor at a dilution equivalent to 1:40 unextracted serum, reduced maximal insulin-stimulated lipogenesis by 33%, 14C-6-glucose oxidation by 70% and 14C-1-glucose oxidation by 25%. In contrast, insulin-stimulated 2-deoxy-D-(1-3H) glucose uptake was unaffected. In the presence of the inhibitor, insulin sensitivity (insulin dose required for half-maximal response) was unchanged for lipogenesis but decreased for 14C-6-glucose oxidation and increased for 14C-1-glucose oxidation. In the presence of control sera, the low Mr inhibitor decreased basal lipogenesis (a measure of serum insulin-like activity) and its effect on insulin-stimulated lipogenesis was unaltered.(ABSTRACT TRUNCATED AT 250 WORDS)" @default.
- W2416727787 created "2016-06-24" @default.
- W2416727787 creator A5039927596 @default.
- W2416727787 creator A5041815521 @default.
- W2416727787 creator A5090073956 @default.
- W2416727787 date "1987-06-01" @default.
- W2416727787 modified "2023-09-23" @default.
- W2416727787 title "Isolation of a post-binding inhibitor of insulin action from the sera of non-insulin-dependent diabetics." @default.
- W2416727787 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3652616" @default.
- W2416727787 hasPublicationYear "1987" @default.
- W2416727787 type Work @default.
- W2416727787 sameAs 2416727787 @default.
- W2416727787 citedByCount "0" @default.
- W2416727787 crossrefType "journal-article" @default.
- W2416727787 hasAuthorship W2416727787A5039927596 @default.
- W2416727787 hasAuthorship W2416727787A5041815521 @default.
- W2416727787 hasAuthorship W2416727787A5090073956 @default.
- W2416727787 hasConcept C126322002 @default.
- W2416727787 hasConcept C134018914 @default.
- W2416727787 hasConcept C141359234 @default.
- W2416727787 hasConcept C185592680 @default.
- W2416727787 hasConcept C2778024521 @default.
- W2416727787 hasConcept C2779306644 @default.
- W2416727787 hasConcept C555293320 @default.
- W2416727787 hasConcept C62231903 @default.
- W2416727787 hasConcept C71924100 @default.
- W2416727787 hasConceptScore W2416727787C126322002 @default.
- W2416727787 hasConceptScore W2416727787C134018914 @default.
- W2416727787 hasConceptScore W2416727787C141359234 @default.
- W2416727787 hasConceptScore W2416727787C185592680 @default.
- W2416727787 hasConceptScore W2416727787C2778024521 @default.
- W2416727787 hasConceptScore W2416727787C2779306644 @default.
- W2416727787 hasConceptScore W2416727787C555293320 @default.
- W2416727787 hasConceptScore W2416727787C62231903 @default.
- W2416727787 hasConceptScore W2416727787C71924100 @default.
- W2416727787 hasIssue "2" @default.
- W2416727787 hasLocation W24167277871 @default.
- W2416727787 hasOpenAccess W2416727787 @default.
- W2416727787 hasPrimaryLocation W24167277871 @default.
- W2416727787 hasRelatedWork W1966225706 @default.
- W2416727787 hasRelatedWork W2010484394 @default.
- W2416727787 hasRelatedWork W2010817550 @default.
- W2416727787 hasRelatedWork W2032040801 @default.
- W2416727787 hasRelatedWork W2048226041 @default.
- W2416727787 hasRelatedWork W2056971132 @default.
- W2416727787 hasRelatedWork W2078141101 @default.
- W2416727787 hasRelatedWork W2096420073 @default.
- W2416727787 hasRelatedWork W2130981205 @default.
- W2416727787 hasRelatedWork W69781604 @default.
- W2416727787 hasVolume "5" @default.
- W2416727787 isParatext "false" @default.
- W2416727787 isRetracted "false" @default.
- W2416727787 magId "2416727787" @default.
- W2416727787 workType "article" @default.