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- W2416897777 abstract "Synthetic quorum-sensing systems in mammalian cells has enabled the implementation of time- and distance-dependent bioprocesses, as well as the design of synthetic ecosystems emulating clinically important host–parasite interactions. In this chapter, we provide a detailed protocol of the design of a mammalian cell-to-cell signaling device and its integration into a mammalian quorum-sensing system for cell density-induced expression product genes. Cell-to-cell signaling is based on a sender cell, metabolically engineered for expression of alcohol dehydrogenase converting ethanol into acetaldehyde, and a receiver cell line for the dose-dependent translation of the acetaldehyde concentration into transgene expression by an acetaldehyde-responsive promoter. This protocol can be adapted easily to various cell types and transgenes for the design of versatile mammalian cell-based quorum-sensing systems." @default.
- W2416897777 created "2016-06-24" @default.
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- W2416897777 date "2010-10-08" @default.
- W2416897777 modified "2023-09-26" @default.
- W2416897777 title "Design of Synthetic Mammalian Quorum-Sensing Systems" @default.
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- W2416897777 doi "https://doi.org/10.1007/978-1-60761-971-0_17" @default.
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