Matches in SemOpenAlex for { <https://semopenalex.org/work/W2417227788> ?p ?o ?g. }
Showing items 1 to 90 of
90
with 100 items per page.
- W2417227788 endingPage "531" @default.
- W2417227788 startingPage "527" @default.
- W2417227788 abstract "Glu-537 of beta-galactosidase (EC 3.2.1.23) was replaced by Asp, Gln and Val using synthetic oligonucleotides. The kcat values of the purified enzyme mixtures were reduced by about 100-fold for the Asp mutant, 30,000-60,000-fold for the Val mutant and 160,000-300,000-fold for the Gln mutant. The greatest differences in properties from the wild-type enzyme were found for the Asp-substituted enzyme: the Km values increased (from 0.12 to 0.42 mM for o-nitrophenyl beta-D-galactopyranoside), and from 0.04 to 0.37 mM for p-nitrophenyl beta-D-galactopyranoside), the Ki value for isopropyl beta-D-galactopyranoside increased (from 0.11 to 0.30 mM), the stability to heat decreased and methanol did not act as an acceptor. The enzymes with the other two substitutions had properties similar to those of the wild-type. For all three substituted enzymes, the inhibitory effects of the transition-state analogues (2-deoxy-2-amino-D-galactose and L-ribose) and the Mg2+ effects were similar to those of the normal enzyme. As all of the properties (except the kcat values) of the Gln- and Val-substituted enzyme preparations were similar to those of the wild-type enzyme, the activities in those preparations were probably due to the presence of a few wild-type enzyme molecules (formed from misreads) among the substituted enzymes. The enzymes with Gln and Val substitutions appear to be totally inactive. The results obtained support a recent suggestion that Glu-537 is an important catalytic residue of beta-galactosidase." @default.
- W2417227788 created "2016-06-24" @default.
- W2417227788 creator A5040879362 @default.
- W2417227788 creator A5055506413 @default.
- W2417227788 creator A5075411075 @default.
- W2417227788 date "1994-04-15" @default.
- W2417227788 modified "2023-09-27" @default.
- W2417227788 title "Substitutions for Glu-537 of <i>β</i>-galactosidase from <i>Escherichia coli</i> cause large decreases in catalytic activity" @default.
- W2417227788 cites W1506760645 @default.
- W2417227788 cites W1563999032 @default.
- W2417227788 cites W1586196888 @default.
- W2417227788 cites W1605271364 @default.
- W2417227788 cites W1967749712 @default.
- W2417227788 cites W1972956705 @default.
- W2417227788 cites W1979197786 @default.
- W2417227788 cites W1993962769 @default.
- W2417227788 cites W2014117083 @default.
- W2417227788 cites W2018472585 @default.
- W2417227788 cites W2038609470 @default.
- W2417227788 cites W2065905548 @default.
- W2417227788 cites W2070809787 @default.
- W2417227788 cites W2072874324 @default.
- W2417227788 cites W2080647861 @default.
- W2417227788 cites W2888511473 @default.
- W2417227788 doi "https://doi.org/10.1042/bj2990527" @default.
- W2417227788 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1138303" @default.
- W2417227788 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7909660" @default.
- W2417227788 hasPublicationYear "1994" @default.
- W2417227788 type Work @default.
- W2417227788 sameAs 2417227788 @default.
- W2417227788 citedByCount "38" @default.
- W2417227788 countsByYear W24172277882012 @default.
- W2417227788 countsByYear W24172277882013 @default.
- W2417227788 countsByYear W24172277882014 @default.
- W2417227788 countsByYear W24172277882015 @default.
- W2417227788 countsByYear W24172277882016 @default.
- W2417227788 countsByYear W24172277882018 @default.
- W2417227788 countsByYear W24172277882019 @default.
- W2417227788 countsByYear W24172277882020 @default.
- W2417227788 countsByYear W24172277882021 @default.
- W2417227788 crossrefType "journal-article" @default.
- W2417227788 hasAuthorship W2417227788A5040879362 @default.
- W2417227788 hasAuthorship W2417227788A5055506413 @default.
- W2417227788 hasAuthorship W2417227788A5075411075 @default.
- W2417227788 hasBestOaLocation W24172277882 @default.
- W2417227788 hasConcept C104264131 @default.
- W2417227788 hasConcept C104317684 @default.
- W2417227788 hasConcept C143065580 @default.
- W2417227788 hasConcept C155647269 @default.
- W2417227788 hasConcept C181199279 @default.
- W2417227788 hasConcept C185592680 @default.
- W2417227788 hasConcept C207583985 @default.
- W2417227788 hasConcept C41183919 @default.
- W2417227788 hasConcept C55493867 @default.
- W2417227788 hasConcept C56856141 @default.
- W2417227788 hasConcept C71240020 @default.
- W2417227788 hasConceptScore W2417227788C104264131 @default.
- W2417227788 hasConceptScore W2417227788C104317684 @default.
- W2417227788 hasConceptScore W2417227788C143065580 @default.
- W2417227788 hasConceptScore W2417227788C155647269 @default.
- W2417227788 hasConceptScore W2417227788C181199279 @default.
- W2417227788 hasConceptScore W2417227788C185592680 @default.
- W2417227788 hasConceptScore W2417227788C207583985 @default.
- W2417227788 hasConceptScore W2417227788C41183919 @default.
- W2417227788 hasConceptScore W2417227788C55493867 @default.
- W2417227788 hasConceptScore W2417227788C56856141 @default.
- W2417227788 hasConceptScore W2417227788C71240020 @default.
- W2417227788 hasIssue "2" @default.
- W2417227788 hasLocation W24172277881 @default.
- W2417227788 hasLocation W24172277882 @default.
- W2417227788 hasLocation W24172277883 @default.
- W2417227788 hasOpenAccess W2417227788 @default.
- W2417227788 hasPrimaryLocation W24172277881 @default.
- W2417227788 hasRelatedWork W2002102692 @default.
- W2417227788 hasRelatedWork W2013630470 @default.
- W2417227788 hasRelatedWork W2073638121 @default.
- W2417227788 hasRelatedWork W2092972772 @default.
- W2417227788 hasRelatedWork W2124597856 @default.
- W2417227788 hasRelatedWork W2279156773 @default.
- W2417227788 hasRelatedWork W2379610813 @default.
- W2417227788 hasRelatedWork W2417227788 @default.
- W2417227788 hasRelatedWork W258580054 @default.
- W2417227788 hasRelatedWork W214510971 @default.
- W2417227788 hasVolume "299" @default.
- W2417227788 isParatext "false" @default.
- W2417227788 isRetracted "false" @default.
- W2417227788 magId "2417227788" @default.
- W2417227788 workType "article" @default.